| Copper toxicity in gills of the teleost fish, Oreochromis niloticus: effects in apoptosis induction and cell proliferation. | |
| | |
MedLine Citation:
|
PMID: 19656581 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Recent in vitro studies have demonstrated that copper may induce apoptosis triggering the activation of caspase-3, a central effector of apoptotic cell death. However, the precise mechanism of copper-induced apoptosis is still unclear, even less so in Oreochromis niloticus where no caspase genes have been reported so far. This study aimed to assess the in vivo role of copper in apoptosis induction on O. niloticus gill, simultaneously contributing to elucidate the mechanism of copper-induced apoptosis. Caspase-3 gene was partially sequenced and, after in vivo exposures to 40 and 400 microgL(-1) of copper, its mRNA expression was evaluated by real-time PCR. Apoptosis was also evaluated by TUNEL assay and cell proliferation identified using an antibody against proliferating cell nuclear antigen (PCNA). The copper concentrations used did not induce the upregulation of caspase-3 gene in O. niloticus gill. In addition, in the gills of fish exposed to copper there was no increase in the estimated relative volume of apoptotic cells, indicating that neither the caspase-3-dependent or caspase-independent apoptotic pathways were induced. On the other hand, the increase in the volumetric density of epithelial proliferating cells suggests a concentration-dependent repair response. |
| | |
Authors:
|
Sandra Mariza Monteiro; Nuno M S dos Santos; Margarida Calejo; António Fontainhas-Fernandes; Mário Sousa |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-07-16 |
Journal Detail:
|
Title: Aquatic toxicology (Amsterdam, Netherlands) Volume: 94 ISSN: 1879-1514 ISO Abbreviation: Aquat. Toxicol. Publication Date: 2009 Sep |
Date Detail:
|
Created Date: 2009-09-02 Completed Date: 2009-10-21 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8500246 Medline TA: Aquat Toxicol Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 219-28 Citation Subset: IM |
Affiliation:
|
Department of Biology and Environment-CITAB, University of Trás-os-Montes and Alto Douro, Apartado 1013, 5001-801 Vila Real, Portugal. smonteir@utad.pt |
| Data Bank Information | |
Bank Name/Acc. No.:
|
GENBANK/EU887950; EU887951 |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Animals Apoptosis* Base Sequence Caspase 3 / genetics, metabolism* Cell Proliferation / drug effects Cichlids / genetics, metabolism* Copper / toxicity* Gills / drug effects*, enzymology, metabolism Molecular Sequence Data RNA, Messenger / metabolism Water Pollutants, Chemical / toxicity* |
| Chemical | |
Reg. No./Substance:
|
0/RNA, Messenger; 0/Water Pollutants, Chemical; 7440-50-8/Copper; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Mycophenolate mofetil as a treatment of steroid dependent Cogan's syndrome in childhood.
Next Document: Kinetics and effects of dichloroacetic acid in rainbow trout.