| Copper sensitivity of cueO mutants of Escherichia coli K-12 and the biochemical suppression of this phenotype. | |
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MedLine Citation:
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PMID: 15708004 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The cueO gene of Escherichia coli encodes a multi-copper oxidase, which contributes to copper tolerance in this bacterium. It was observed that a cueO mutant was highly sensitive to killing by copper ions when cells were grown on defined minimal media. Copper sensitivity was correlated with accumulation of copper in the mutant strain. Growth of the cueO mutant in the presence of copper could be restored by addition of divalent zinc and manganese ions or ferrous iron but not by other first row transition metal ions or magnesium ions. Copper toxicity towards a cueO mutant could also be suppressed by addition of the superoxide quencher 1,2-dihydroxybenzene-3,5-disulfonic acid (tiron), suggesting that a primary cause of copper toxicity is the copper-catalyzed production of superoxide anions in the cytoplasm. |
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Authors:
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Jai J Tree; Stephen P Kidd; Michael P Jennings; Alastair G McEwan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 328 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2005 Mar |
Date Detail:
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Created Date: 2005-02-14 Completed Date: 2005-04-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 1205-10 Citation Subset: IM |
Affiliation:
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Centre for Metals in Biology, School of Molecular and Microbial Sciences, University of Queensland, Brisbane 4072, Australia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Survival
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drug effects Copper / pharmacokinetics*, pharmacology* Dose-Response Relationship, Drug Drug Resistance, Microbial / physiology Escherichia coli K12 / cytology, drug effects*, genetics, metabolism* Escherichia coli Proteins Metals Mutation Oxidoreductases / genetics, metabolism* Phenotype Sensitivity and Specificity Species Specificity Transition Elements / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Escherichia coli Proteins; 0/Metals; 0/Transition Elements; 7440-50-8/Copper; EC 1.-/Oxidoreductases; EC 1.-/cueO protein, E coli |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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