Document Detail

Copper may interact with selenite extracellularly in cultured HT-29 cells.
MedLine Citation:
PMID:  15023400     Owner:  NLM     Status:  MEDLINE    
Previous studies have demonstrated that copper (15.7 micromol/L) can inhibit selenite (12.6 micromol/L)-induced cytotoxicity and apoptosis in HT-29 cells. However, the exact nature of the interactions between selenium and copper is not fully understood. In this study, the effect of copper on the cell cycle arrest induced by selenite or selenocystine was examined. Both selenite and selenocystine were effective in inhibition of cell growth and cell cycle progression. Cell cycle analysis revealed that selenite (3-5 micromol/L) caused a decrease in G1 phase cells that corresponded with an increase in S and G2 phase cells, and that 0.625 or 1.25 micromol/L copper sufficiently inhibited selenite-induced cell cycle arrest. In contrast, selenocystine caused an increase in G1 phase cells that corresponded with a decrease in S and G2 phase cells. Interestingly, 0.625 or 1.25 micromol/L copper did not inhibit selenocystine-induced cell cycle arrest. In addition, cell free gel shift assay demonstrated that selenite suppressed the inhibitory effect of copper on SP-1 DNA binding. Furthermore, although 5 micromol/L selenite in culture media significantly increased the intracellular selenium content, 1.25 micromol/L copper sulfate blocked this increase of the intracellular selenium content. Collectively, these data demonstrate that selenite and selenocystine cause cell cycle arrest via distinct mechanisms, and suggest that copper may interact with selenite extracellularly, which represents the basis of antagonism between copper sulfate and selenite.
Huawei Zeng; James H Botnen
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of nutritional biochemistry     Volume:  15     ISSN:  0955-2863     ISO Abbreviation:  J. Nutr. Biochem.     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-03-16     Completed Date:  2004-10-08     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9010081     Medline TA:  J Nutr Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  179-84     Citation Subset:  IM    
United States Department of Agriculture, Agricultural Research Service, Grand Forks Human Nutrition Research Center, PO Box 9034, Grand Forks, ND 58202-9034, USA.
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MeSH Terms
Cell Cycle
Cell Division
Cell Line, Tumor
Cell Nucleus / metabolism
Cell-Free System
Copper / chemistry,  metabolism*,  pharmacology
Copper Sulfate / pharmacology
DNA / metabolism
Dose-Response Relationship, Drug
G1 Phase
G2 Phase
S Phase
Selenocysteine / pharmacology
Sodium Selenite / metabolism*
Reg. No./Substance:
10102-18-8/Sodium Selenite; 10236-58-5/Selenocysteine; 7440-50-8/Copper; 7758-98-7/Copper Sulfate; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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