Document Detail


Copper induction of enhanced green fluorescent protein expression in Pleurotus ostreatus driven by laccase poxa1b promoter.
MedLine Citation:
PMID:  23050832     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
In silico analyses of several laccase promoter sequences have shown the presence of many different responsive elements differentially distributed along the promoter sequences. Analysis of P. ostreatus laccase promoter poxa1b extending around 1400-bp upstream of the start codon showed the presence of several putative response elements, such as ten Metal Responsive Elements. Development of a system for in vivo analysis of P. ostreatus laccase promoter poxa1b by enhanced green fluorescent protein expression was carried out, based on a poly ethylene glycol mediated procedure for fungal transformation. Quantitative measurement of fluorescence expressed in P. ostreatus transformants grown in the presence and in the absence of copper sulphate was performed, demonstrating an increase of expression level induced by the metal. © 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd.All rights reserved.
Authors:
Antonella Amore; Yoichi Honda; Vincenza Faraco
Publication Detail:
Type:  LETTER     Date:  2012-10-11
Journal Detail:
Title:  FEMS microbiology letters     Volume:  -     ISSN:  1574-6968     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Affiliation:
Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte S. Angelo, via Cintia, 4 80126, Naples, Italy.
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