| Copper-induced oxidative damage on astrocytes: protective effect exerted by human high density lipoproteins. | |
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MedLine Citation:
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PMID: 14642776 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In the present study, we confirmed that copper ions induce oxidative damage in human astrocytes in culture, as demonstrated by the significant increase in the levels of hydroperoxides and in the fluorescence intensity of the fluorescent probe dichloro-dihydrofluorescein diacetate (H(2)DCFDA). The compositional changes were associated with a significant decrease in cell viability in astrocytes treated with 10 microM Cu(++) with respect to control cells. Astrocytes incubated with copper ions in the presence of high density lipoproteins (HDL) isolated from plasma of normolipemic subjects showed lower levels of hydroperoxides and a higher cell viability with respect to cells oxidized alone. Moreover, a significant decrease in the levels of hydroperoxides was observed in oxidized astrocytes treated with HDL. These results demonstrate that HDL exert a protective role against lipid peroxidation. The protective effect could be related to the ability of HDL to bind metal ions at the lipoprotein surface and/or to a stimulation of the efflux of lipid hydroperoxides from cell membranes as demonstrated in other cell types. Oxidative damage of astrocytes was induced at a copper concentration similar to that observed in cerebrospinal fluid (CSF) of patients affected by neurodegenerative diseases such as Alzheimer's (AD) and Parkinson's diseases (PD). Lipoprotein particles similar for density and chemical composition to plasma HDL were recently isolated in human CSF, therefore, the protective role exerted by HDL against Cu(++)-induced oxidative damage of astrocytes could be of physiological relevance. |
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Authors:
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Gianna Ferretti; Tiziana Bacchetti; Cinzia Moroni; Arianna Vignini; Giovanna Curatola |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1635 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 2003 Nov |
Date Detail:
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Created Date: 2003-12-03 Completed Date: 2004-01-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 48-54 Citation Subset: IM |
Affiliation:
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Istituto di Biochimica, Facoltà of Medicina, Università Politecnica delle Marche, Via Ranieri, I-60131 Ancona, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Astrocytes
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cytology*,
drug effects,
pathology Astrocytoma Cell Survival / drug effects Cells, Cultured Copper / toxicity* Copper Sulfate / toxicity* Humans Kinetics Lipid Peroxidation / drug effects* Lipoproteins, HDL / blood, pharmacology* Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Lipoproteins, HDL; 7440-50-8/Copper; 7758-98-7/Copper Sulfate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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