| Copper exposure effects on yeast mitochondrial proteome. | |
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MedLine Citation:
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PMID: 21549866 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Mitochondria play an important role on the entire cellular copper homeostatic mechanisms. Alteration of cellular copper levels may thus influence mitochondrial proteome and its investigation represents an important contribution to the general understanding of copper-related cellular effects. In these study we have performed an organelle targeted proteomic investigation focusing our attention on the effect of non-lethal 1mM copper concentration on Saccharomyces cerevisiae mitochondrial proteome. Functional copper effects on yeast mitochondrial proteome were evaluated by using both 2D electrophoresis (2-DE) and liquid chromatography coupled with tandem mass spectrometry. Proteomic data have been then analyzed by different unsupervised meta-analysis approaches that highlight the impairment of mitochondrial functions and the activation of oxidative stress response. Interestingly, our data have shown that stress response generated by 1mM copper treatment determines the activation of S. cerevisiae survival pathway. To investigate these findings we have treated yeast cells responsiveness to copper with hydrogen peroxide and observed a protective role of this metal. These results are suggestive of a copper role in the protection from oxidative stress possibly due to the activation of mechanisms involved in cellular survival and growth. |
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Authors:
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Lucia Banci; Ivano Bertini; Simone Ciofi-Baffoni; Annamaria D'Alessandro; Deepa Jaiswal; Valeria Marzano; Sara Neri; Maurizio Ronci; Andrea Urbani |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-4-23 |
Journal Detail:
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Title: Journal of proteomics Volume: - ISSN: 1876-7737 ISO Abbreviation: - Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-5-9 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101475056 Medline TA: J Proteomics Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011. Published by Elsevier B.V. |
Affiliation:
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Magnetic Resonance Center CERM and Department of Chemistry, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Florence, Italy. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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