Document Detail

Copper-GHK increases integrin expression and p63 positivity by keratinocytes.
MedLine Citation:
PMID:  19319546     Owner:  NLM     Status:  MEDLINE    
Glycyl-L-histidyl-L-lysyl (GHK) possesses a high affinity for copper(II) ions, with which it spontaneously forms a complex (copper-GHK). It is well known that copper-GHK plays a physiological role in the process of wound healing and tissue repair by stimulating collagen synthesis in fibroblasts. This study was conducted to investigate the effects of copper-GHK on keratinocytes. Proliferative effects were analyzed and hematoxylin and eosin staining and immunohistochemistry were conducted to evaluate the effects of copper-GHK in skin equivalent (SE) models. In addition, western blotting was performed. In monolayer cultured keratinocytes, copper-GHK increased the proliferation of keratinocytes. When the SE models were evaluated, basal cells became cuboidal when copper-GHK was added. Immunohistochemical analysis revealed that copper-GHK increased proliferating cell nuclear antigen (PCNA) and p63 positivity. Furthermore, the expression of integrin alpha6 and beta1 increased in SE models, and these results were confirmed by Western blotting. The results of this study indicate that treatment with copper-GHK may increase the proliferative potential of basal keratinocytes by modulating the expression of integrins, p63 and PCNA. In addition, increased levels of p63, a putative stem cell marker of the skin, suggests that copper-GHK promotes the survival of basal stem cells in the skin.
Youn-A Kang; Hye-Ryung Choi; Jung-Im Na; Chang-Hun Huh; Min-Ji Kim; Sang-Woong Youn; Kyu-Han Kim; Kyoung-Chan Park
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-03-25
Journal Detail:
Title:  Archives of dermatological research     Volume:  301     ISSN:  1432-069X     ISO Abbreviation:  Arch. Dermatol. Res.     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-05-01     Completed Date:  2009-07-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8000462     Medline TA:  Arch Dermatol Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  301-6     Citation Subset:  IM    
Department of Dermatology, Seoul National University College of Medicine, Yeongeon-dong, Jongno-gu, Seoul, Republic of Korea.
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MeSH Terms
Antigens, CD29 / genetics,  metabolism*
Cell Proliferation / drug effects
Copper / chemistry*,  metabolism
Foreskin / cytology
Infant, Newborn
Integrin alpha6 / genetics,  metabolism*
Keratinocytes / cytology,  drug effects,  metabolism*
Membrane Proteins / genetics,  metabolism*
Oligopeptides / metabolism*
Organ Culture Techniques
Organometallic Compounds / pharmacology*
Proliferating Cell Nuclear Antigen / genetics,  metabolism
Protein Binding
Protein Conformation
Tumor Suppressor Protein p53 / genetics,  metabolism
Reg. No./Substance:
0/Antigens, CD29; 0/CKAP4 protein, human; 0/Integrin alpha6; 0/Membrane Proteins; 0/Oligopeptides; 0/Organometallic Compounds; 0/Proliferating Cell Nuclear Antigen; 0/Tumor Suppressor Protein p53; 7440-50-8/Copper

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