| Copine A is expressed in prestalk cells and regulates slug phototaxis and thermotaxis in developing Dictyostelium. | |
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MedLine Citation:
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PMID: 21950343 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Copines are calcium-dependent membrane-binding proteins found in many eukaryotic organisms. We are studying the function of copines using the model organism, Dictyostelium discoideum. When under starvation conditions, Dictyostelium cells aggregate into mounds that become migrating slugs, which can move toward light and heat before culminating into a fruiting body. Previously, we showed that Dictyostelium cells lacking the copine A (cpnA) gene are not able to form fruiting bodies and instead arrest at the slug stage. In this study, we compared the slug behavior of cells lacking the cpnA gene to the slug behavior of wild-type cells. The slugs formed by cpnA- cells were much larger than wild-type slugs and exhibited no phototaxis and negative thermotaxis in the same conditions that wild-type slugs exhibited positive phototaxis and thermotaxis. Mixing as little as 5% wild-type cells with cpnA- cells rescued the phototaxis and thermotaxis defects, suggesting that CpnA plays a specific role in the regulation of the production and/or release of a signaling molecule. Reducing extracellular levels of ammonia also partially rescued the phototaxis and thermotaxis defects of cpnA- slugs, suggesting that CpnA may have a specific role in regulating ammonia signaling. Expressing the lacZ gene under the cpnA promoter in wild-type cells indicated cpnA is preferentially expressed in the prestalk cells found in the anterior part of the slug, which include the cells at the tip of the slug that regulate phototaxis, thermotaxis, and the initiation of culmination into fruiting bodies. Our results suggest that CpnA plays a role in the regulation of the signaling pathways, including ammonia signaling, necessary for sensing and/or orienting toward light and heat in the prestalk cells of the Dictyostelium slug. |
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Authors:
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Kerry A Flegel; Jaimie M Pineda; Tasha S Smith; Ann M Laszczyk; Janet M Price; Kristen M Karasiewicz; Cynthia K Damer |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-09-23 |
Journal Detail:
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Title: Development, growth & differentiation Volume: 53 ISSN: 1440-169X ISO Abbreviation: Dev. Growth Differ. Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-10-17 Completed Date: 2012-02-01 Revised Date: 2013-05-23 |
Medline Journal Info:
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Nlm Unique ID: 0356504 Medline TA: Dev Growth Differ Country: Japan |
Other Details:
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Languages: eng Pagination: 948-59 Citation Subset: IM |
Copyright Information:
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© 2011 The Authors. Development, Growth & Differentiation © 2011 Japanese Society of Developmental Biologists. |
Affiliation:
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Biology Department, Central Michigan University, Mount Pleasant, Michigan 48859, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carrier Proteins / genetics*, metabolism, physiology* Cells, Cultured Dictyostelium / cytology, genetics, growth & development*, physiology Gene Expression Regulation, Developmental Lac Operon Life Cycle Stages / genetics* Light Movement* / physiology, radiation effects Organisms, Genetically Modified Promoter Regions, Genetic Temperature Tissue Distribution |
| Grant Support | |
ID/Acronym/Agency:
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1R15GM078089-01A1/GM/NIGMS NIH HHS; R15 GM078089-01A1/GM/NIGMS NIH HHS; R15 GM078089-01A1S1/GM/NIGMS NIH HHS; R15 GM078089-01A1S2/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/copine |
| Comments/Corrections | |
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