Document Detail


Coordination structures of Mg(2+) and Ca(2+) in three types of tobacco calmodulins in solution: Fourier-transform infrared spectroscopic studies of side-chain COO(-) groups.
MedLine Citation:
PMID:  23616215     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Calmodulin (CaM) is a Ca(2+) -binding protein that regulates a number of fundamental cellular activities. Nicotiana tabacum CaM (NtCaM) comprises 13 genes classified into three types, among which gene expression and target enzyme activation differ. We performed Fourier-transform infrared spectroscopy to compare the secondary and coordination structures of Mg(2+) and Ca(2+) among NtCaM1, NtCaM3, and NtCaM13 as representatives of the three types of NtCaMs. Data suggested that NtCaM13 has a different secondary structure due to the weak β-strand bands and the weak 1661 cm(-1) band. Coordination structures of Mg(2+) of NtCaM3 and NtCaM13 were similar but different from that of NtCaM1, while the Ca(2+) -binding manner was similar among the three CaMs. The amplitude differences of the band at 1554-1550 cm(-1) obtained by second-derivative spectra indicated that the intensity change of the band of NtCaM13 was smaller in response to [Ca(2+) ] increases under low [Ca(2+) ] conditions than were those of NtCaM1 and NtCaM3, while the intensity reached the same level under high [Ca(2+) ]. Therefore, NtCaM13 has a characteristic secondary structure and specific Mg(2+) -binding manner and needs higher [Ca(2+) ] for bidentate Ca(2+) coordination of 12th Glu in EF-hand motifs. The Ca(2+) -binding mechanisms of the EF-hand motifs of the three CaMs are similar; however, the cation-dependent conformational change in NtCaM13 is unique among the three NtCaMs. © 2013 Wiley Periodicals, Inc. Biopolymers 99: 472-483, 2013.
Authors:
Nanao Suzuki; Lica Fabiana Imai; Yusuke Kato; Koji Nagata; Yuko Ohashi; Kazuyuki Kuchitsu; Masaru Tanokura; Akira Sakamoto; Masayuki Nara; Minoru Nakano; Naoto Yonezawa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biopolymers     Volume:  99     ISSN:  0006-3525     ISO Abbreviation:  Biopolymers     Publication Date:  2013 Jul 
Date Detail:
Created Date:  2013-04-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372525     Medline TA:  Biopolymers     Country:  United States    
Other Details:
Languages:  eng     Pagination:  472-83     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Wiley Periodicals, Inc.
Affiliation:
Department of Chemistry, Graduate School of Science, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba-shi, Chiba, 263-8522, Japan; Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo, 113-8657, Japan.
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