Document Detail


Coordination of mitochondrial bioenergetics with G1 phase cell cycle progression.
MedLine Citation:
PMID:  18583942     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Relatively little is known regarding how energetic demand during cell proliferation is sensed or coordinated with mitochondrial metabolism. Here we demonstrate that cell cycle progression through G(1) is associated with a significant increase in mitochondrial membrane potential (DeltaPsi(m)) and respiration. We used this change in metabolic rate to isolate cells in G(1) with low and high levels of mitochondrial membrane potential (DeltaPsi(m)L and DeltaPsi(m)H). Biochemical and functional studies demonstrate that DeltaPsi(m)L and DeltaPsi(m)H cells display the distinct characteristics of early and late G(1) phase, respectively. We further demonstrate that the metabolic rate in G(1) reflects levels of the mTOR-raptor complex as well as susceptibility to rapamycin-induced cell cycle delay. In conclusion, our data suggests a coupling of mitochondrial bioenergetics and G(1) progression and points to the mTOR signaling pathway as a potential molecular coordinator of these two processes.
Authors:
Stefan M Schieke; J Philip McCoy; Toren Finkel
Publication Detail:
Type:  Journal Article     Date:  2008-06-02
Journal Detail:
Title:  Cell cycle (Georgetown, Tex.)     Volume:  7     ISSN:  1551-4005     ISO Abbreviation:  Cell Cycle     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-08-27     Completed Date:  2008-10-07     Revised Date:  2012-09-25    
Medline Journal Info:
Nlm Unique ID:  101137841     Medline TA:  Cell Cycle     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1782-7     Citation Subset:  IM    
Affiliation:
Translational Medicine Branch, NHLBI, NIH, Bethesda, Maryland 20892-1622, USA.
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MeSH Terms
Descriptor/Qualifier:
Adaptor Proteins, Signal Transducing
Cell Line
Cell Respiration
Energy Metabolism
G1 Phase*
Humans
Jurkat Cells
Membrane Potential, Mitochondrial
Mitochondria / metabolism*
Protein Kinases
Proteins / metabolism
TOR Serine-Threonine Kinases
Grant Support
ID/Acronym/Agency:
Z99 HL999999/HL/NHLBI NIH HHS; ZIC HL005905-02/HL/NHLBI NIH HHS; ZIC HL005905-03/HL/NHLBI NIH HHS; ZIC HL005905-04/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Adaptor Proteins, Signal Transducing; 0/Proteins; 0/RPTOR protein, human; EC 2.7.-/Protein Kinases; EC 2.7.1.1/MTOR protein, human; EC 2.7.1.1/TOR Serine-Threonine Kinases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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