| Coordination between donor cell type and cell cycle stage improves nuclear cloning efficiency in cattle. | |
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MedLine Citation:
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PMID: 12499017 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Several studies have shown that both quiescent and proliferating somatic donor cells can be fully reprogrammed after nuclear transfer (NT) and result in viable offspring. So far, however, no comparative study has conclusively demonstrated the relative importance of donor cell cycle stage on nuclear cloning efficiency. Here, we compare two different types of bovine fetal fibroblasts (BFFs) that were synchronized in G(0), G(1), and different phases within G(1). We show that for non-transgenic (non-TG) fibroblasts, serum starvation into G(0) results in a significantly higher percentage of viable calves at term than synchronization in early G(1) or late G(1). For transgenic fibroblasts, however, cells selected in G(1) show significantly higher development to calves at term and higher post-natal survival to weaning than cells in G(0). This suggests that it may be necessary to coordinate donor cell type and cell cycle stage to maximize overall cloning efficiency. |
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Authors:
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D N Wells; G Laible; F C Tucker; A L Miller; J E Oliver; T Xiang; J T Forsyth; M C Berg; K Cockrem; P J L'Huillier; H R Tervit; B Oback |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Theriogenology Volume: 59 ISSN: 0093-691X ISO Abbreviation: Theriogenology Publication Date: 2003 Jan |
Date Detail:
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Created Date: 2002-12-24 Completed Date: 2003-03-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0421510 Medline TA: Theriogenology Country: United States |
Other Details:
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Languages: eng Pagination: 45-59 Citation Subset: IM |
Copyright Information:
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Copyright 2002 Elsevier Science Inc. |
Affiliation:
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Reproductive Technologies, AgResearch Ltd, Ruakura Research Centre, East Street, Private Bag 3123, Hamilton, New Zealand. david.wells@agresearch.co.nz |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle / embryology, genetics* Cell Cycle* Cloning, Organism* Embryo Transfer / veterinary Embryonic and Fetal Development Female Fibroblasts / ultrastructure G0 Phase G1 Phase G2 Phase Mitosis Nuclear Transfer Techniques* Pregnancy |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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