Document Detail


Coordination sphere tuning of the electron transfer dissociation behavior of Cu(II)-peptide complexes.
MedLine Citation:
PMID:  22161629     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In contrast to previous electron capture dissociation (ECD) studies, we find that electron transfer dissociation (ETD) of Cu(II)-peptide complexes can generate c- and z-type product ions when the peptide has a sufficient number of strongly coordinating residues. Double-resonance experiments, ion-molecule reactions, and collision-induced dissociation (CID) prove that the c and z product ions are formed via typical radical pathways without the associated reduction of Cu(II), despite the high second ionization energy of Cu. A positive correlation between the number of Cu(II) binding groups in the peptide sequence and the extent of c and z ion formation was also observed. This trend is rationalized by considering that the recombination energy of Cu(II) can be lowered by strong binding ligands to an extent that enables electron transfer to non-Cu sites (e.g., protonation sites) to compete with Cu(II) reduction, thereby generating c/z ions in a manner similar to that observed for protonated (i.e., nonmetalated) peptides.
Authors:
Jia Dong; Richard W Vachet
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-12-09
Journal Detail:
Title:  Journal of the American Society for Mass Spectrometry     Volume:  23     ISSN:  1879-1123     ISO Abbreviation:  J. Am. Soc. Mass Spectrom.     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-01-24     Completed Date:  2012-03-28     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  9010412     Medline TA:  J Am Soc Mass Spectrom     Country:  United States    
Other Details:
Languages:  eng     Pagination:  321-9     Citation Subset:  IM    
Copyright Information:
© American Society for Mass Spectrometry, 2011
Affiliation:
Department of Chemistry, University of Massachusetts, LGRT 701, 10 N. Pleasant St., Amherst, MA 01003, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Coordination Complexes / chemistry*
Copper / chemistry*
Molecular Sequence Data
Peptides / chemistry*
Spectrometry, Mass, Electrospray Ionization
Tandem Mass Spectrometry / methods*
Grant Support
ID/Acronym/Agency:
R01 GM 075092/GM/NIGMS NIH HHS; R01 GM075092-07/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Coordination Complexes; 0/Peptides; 7440-50-8/Copper
Comments/Corrections

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