Document Detail


Coordination of intercellular Ca(2+) signaling in endothelial cell tubes of mouse resistance arteries.
MedLine Citation:
PMID:  22860994     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To test the hypothesis that Ca(2+) responses to GPCR activation are coordinated between neighboring ECs of resistance arteries.
METHODS: EC tubes were freshly isolated from superior epigastric arteries of C57BL/6 mice. Intercellular coupling was tested using microinjection of propidium iodide. Following loading with fluo-4 dye, intracellular Ca(2+) responses to ACh were imaged with confocal microscopy.
RESULTS: Cell-to-cell transfer of propidium iodide confirmed functional GJCs. A 1 μm ACh stimulus evoked Ca(2+) responses (9.8 ± 0.8/min, F/F(0) = 3.11 ± 0.2) which pseudo-line-scan analysis revealed as composed of Ca(2+) waves and spatially restricted Ca(2+) release events. A 100 nm ACh stimulus induced Ca(2+) responses of lower frequency (4.5 ± 0.7/min) and amplitude (F/F(0) = 1.95 ± 0.11) composed primarily of spatially restricted events. The time interval between Ca(2+) waves in adjacent cells (0.79 ± 0.12 s) was shorter (p < 0.05) than that between nonadjacent cells (1.56 ± 0.25 s). Spatially restricted Ca(2+) release events had similar frequencies and latencies between adjacent and nonadjacent cells. Inhibiting intracellular Ca(2+) release with 2-APB, Xestospongin C or thapsigargin eliminated Ca(2+) responses.
CONCLUSIONS: With moderate GPCR stimulation, localized Ca(2+) release events predominate among cells. Greater GPCR stimulation evokes coordinated intercellular Ca(2+) waves via the ER. Calcium signaling during GPCR activation is complex among cells, varying with stimulus intensity and proximity to actively signaling cells.
Authors:
Matthew J Socha; Timothy L Domeier; Erik J Behringer; Steven S Segal
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Microcirculation (New York, N.Y. : 1994)     Volume:  19     ISSN:  1549-8719     ISO Abbreviation:  Microcirculation     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-20     Completed Date:  2013-05-03     Revised Date:  2013-11-06    
Medline Journal Info:
Nlm Unique ID:  9434935     Medline TA:  Microcirculation     Country:  United States    
Other Details:
Languages:  eng     Pagination:  757-70     Citation Subset:  IM    
Copyright Information:
© 2012 John Wiley & Sons Ltd.
Affiliation:
Department of Medical Pharmacology and Physiology, University of Missouri, Columbia, Missouri 65212, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcium Signaling / drug effects,  physiology*
Endothelial Cells / metabolism*
Enzyme Inhibitors / pharmacology
Macrocyclic Compounds / pharmacology
Male
Mice
Oxazoles / pharmacology
Thapsigargin / pharmacology
Vascular Resistance / drug effects,  physiology*
Grant Support
ID/Acronym/Agency:
F32-HL107050/HL/NHLBI NIH HHS; R01 HL086483/HL/NHLBI NIH HHS; R01-HL086483/HL/NHLBI NIH HHS; R37 HL041026/HL/NHLBI NIH HHS; R37-HL041026/HL/NHLBI NIH HHS; T32-AR048523/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Macrocyclic Compounds; 0/Oxazoles; 0/xestospongin C; 67526-95-8/Thapsigargin
Comments/Corrections
Erratum In:
Microcirculation. 2013 Jan;20(1):115

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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