Document Detail


Coordinates, DNA content and heterogeneity of cell nuclei and segments of the Caenorhabditis elegans intestine.
MedLine Citation:
PMID:  16158289     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A specific protocol allowed to selectively stain and image the cell nuclei of the intestine of the nematode Caenorhabditis elegans. Digital processing of these images yielded quantitative information on the coordinates and the relative DNA content of these cell nuclei. With this technique, the exact locations (in relation to the length of worm or intestine) of the 20 intestinal cell nuclei of larval stage L1 and of the 34 intestinal cell nuclei of larval stages L2-4 and adults were determined. Tracking the DNA content of an individual intestinal cell nuclei allowed to study nuclear DNA endoreduplications during larval growth. The evaluation of the DNA content of all intestinal cell nuclei revealed a strong heterogeneity between the cell nuclei and intestinal segments with a maximum in segments int4-int5 in the L1 stage and int5-int6 in the L2-L4 stages. Minimum values were detected in the segments int2 and int8-int9 of all larval stages. Heterogeneity between the intestinal segments of C. elegans does not only concern the DNA content of cell nuclei, but also other cellular features like the quantity of intracellular vesicles or the absorption of particles out of the intestinal lumen.
Authors:
Marc Wolf; Frank Nunes; Rüdiger J Paul
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Publication Detail:
Type:  Journal Article     Date:  2005-11-03
Journal Detail:
Title:  Histochemistry and cell biology     Volume:  124     ISSN:  0948-6143     ISO Abbreviation:  Histochem. Cell Biol.     Publication Date:  2005 Nov 
Date Detail:
Created Date:  2005-11-03     Completed Date:  2005-12-20     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9506663     Medline TA:  Histochem Cell Biol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  359-67     Citation Subset:  IM    
Affiliation:
Institut für Zoophysiologie, Westfälische Wilhelms-Universität Münster, Hindenburgplatz 55, 48143, Münster, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bisbenzimidazole / chemistry
Caenorhabditis elegans / physiology*
Cell Division
Cell Nucleus / chemistry*
DNA, Helminth / analysis*,  biosynthesis
Fluorescent Dyes / chemistry
Image Processing, Computer-Assisted / methods
Intestines / chemistry*,  cytology
Larva
Microscopy, Video
Chemical
Reg. No./Substance:
0/DNA, Helminth; 0/Fluorescent Dyes; 23491-44-3/Bisbenzimidazole

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