| Coordinated secretion of alkaline phosphatase into serum and intestine in fat-fed rats. | |
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MedLine Citation:
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PMID: 15599000 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Fat feeding increases the activity of intestinal alkaline phosphatase in the serum. The mechanism underlying this increase is unknown. Surfactant-like particles (SLP) secreted by enterocytes have been implicated in this phenomenon. OBJECTIVE: To study the effect of feeding fish oil and protein synthesis inhibitors on alkaline phosphatase activity in serum and in different intestinal fractions. METHODS: Male albino rats were fed 2 mL of fish oil and were injected cycloheximide or actinomycin D. Alkaline phosphatase activity was determined in the serum and intestinal fractions (SLP, mucosa, muscularis). RESULT: Feeding fish oil significantly elevated alkaline phosphatase activity in serum (p< 0.001) and intestinal mucosa (p< 0.01). Administration of cycloheximide or actinomycin D significantly reduced alkaline phosphatase activity in serum (p< 0.01) and in intestinal mucosa (p< 0.05). BCIP staining of brush border alkaline phosphatase activity in acrylamide gels yielded similar results. CONCLUSIONS: These findings suggest a co-ordination between alkaline phosphatase synthesis and its assembly into lipoprotein vesicles, such as SLP, secreted by enterocytes in response to fat feeding. |
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Authors:
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R Grewal; Akhtar Mahmood |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Indian journal of gastroenterology : official journal of the Indian Society of Gastroenterology Volume: 23 ISSN: 0254-8860 ISO Abbreviation: Indian J Gastroenterol Publication Date: 2004 Sep-Oct |
Date Detail:
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Created Date: 2004-12-15 Completed Date: 2005-02-01 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8409436 Medline TA: Indian J Gastroenterol Country: India |
Other Details:
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Languages: eng Pagination: 175-7 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Panjab University, Sector 14, Chandigarh-160 014, India. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alkaline Phosphatase
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blood,
secretion* Animals Dietary Fats / metabolism* Disease Models, Animal Gastrointestinal Motility / physiology Intestinal Mucosa / enzymology Intestine, Small / enzymology* Male Metabolic Clearance Rate Probability Rats Rats, Inbred Strains Reference Values Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/Dietary Fats; EC 3.1.3.1/Alkaline Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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