Document Detail

Coordinated regulation of hyaluronan and aggrecan content in the articular cartilage of immobilized and exercised dogs.
MedLine Citation:
PMID:  8877929     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To study the influence of joint loading and immobilization on articular cartilage hyaluronan concentration and histological distribution in the knee joints of young dogs subjected to 11 weeks' immobilization by splinting, and 15 weeks' running exercise at a rate of 40 km/day. METHODS: The amount of hyaluronan in articular cartilage was determined by a competitive binding assay using a biotinylated hyaluronan binding complex (HABC) of aggrecan and link protein. Histologic sections were stained for the localization of hyaluronan with the HABC probe. Extracted proteoglycans were characterized by sodium dodecyl sulfate agarose gel electrophoresis. RESULTS: Immobilization significantly reduced the concentration of hyaluronan in all sites studied (tibial and femoral condyles, patellar surface of femur). The proportion of hyaluronan to total uronic acid (mainly from aggrecan) remained unchanged because of a concurrent decrease in aggrecan. The ratio of hyaluronan and aggrecan remained constant also in runners. The staining pattern of free hyaluronan in the tissue sections and the electrophoretic mobility of the extracted proteoglycans were not affected by the different loading regimes. CONCLUSION: Reduced joint loading due to splint immobilization significantly decreases both hyaluronan and aggrecan in the articular cartilage. The remarkably parallel changes in aggrecan and hyaluronan content suggest that joint loading exerts a coordinated influence on their metabolism.
J Haapala; M J Lammi; R Inkinen; J J Parkkinen; U M Agren; J Arokoski; I Kiviranta; H J Helminen; M I Tammi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of rheumatology     Volume:  23     ISSN:  0315-162X     ISO Abbreviation:  J. Rheumatol.     Publication Date:  1996 Sep 
Date Detail:
Created Date:  1997-01-16     Completed Date:  1997-01-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7501984     Medline TA:  J Rheumatol     Country:  CANADA    
Other Details:
Languages:  eng     Pagination:  1586-93     Citation Subset:  IM; S    
Department of Anatomy, University of Kuopio, Finland.
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MeSH Terms
Binding, Competitive
Cartilage, Articular / metabolism*
Electrophoresis, Polyacrylamide Gel
Extracellular Matrix Proteins*
Glycosaminoglycans / metabolism
Hyaluronic Acid / metabolism*
Lectins, C-Type
Motor Activity*
Osmolar Concentration
Proteoglycans / metabolism*
Time Factors
Tissue Distribution
Uronic Acids / metabolism
Reg. No./Substance:
0/Aggrecans; 0/Extracellular Matrix Proteins; 0/Glycosaminoglycans; 0/Lectins, C-Type; 0/Proteoglycans; 0/Uronic Acids; 58-85-5/Biotin; 9004-61-9/Hyaluronic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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