| Coordinated expression of MMPs and TIMPs in rat knee intra-articular tissues after ACL injury. | |
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MedLine Citation:
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PMID: 19863390 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Anterior cruciate ligament (ACL) has poor healing ability and an injured ACL would induce the degeneration of other intra-articular connective tissues. However, the coordinated expression and activities of matrix metalloproteinase (MMPs) in intra-articular tissues induced by ACL rupture were poorly understood. With a rat ACL rotating injury model, we found that after ACL injury, the mRNA levels of MMP-13, TIMP-1, and CD147 were significantly elevated in ACL, posterior cruciate ligament (PCL), synovium, meniscus, and cartilage. Also, MMP-2 activity was also elevated significantly in a time-dependent manner in all intra-articular tissues. Synovium showed the most capability to release MMPs, whereas ACL showed the highest MMP-13/TIMP-1 ratio. Generic MMP activity assay and zymography showed time dependent elevation of MMP activities in synovial fluids (SF). We concluded that the ACL injury would induce a coordinated response of intra-articular tissues to express MMPs, TIMPs, and CD147. The MMP activities in the microenvironment in SF would accumulate, released by all the intra-articular tissues, which would contribute to the knee damage and degeneration induced by ACL injury. |
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Authors:
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Zhenyu Tang; Li Yang; Jin Zhang; Ruyue Xue; Yequan Wang; Peter C Chen; K-L Paul Sung |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Connective tissue research Volume: 50 ISSN: 1607-8438 ISO Abbreviation: Connect. Tissue Res. Publication Date: 2009 |
Date Detail:
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Created Date: 2009-10-29 Completed Date: 2010-01-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0365263 Medline TA: Connect Tissue Res Country: England |
Other Details:
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Languages: eng Pagination: 315-22 Citation Subset: IM |
Affiliation:
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Chongqing University, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anterior Cruciate Ligament / enzymology*, injuries*, physiopathology Antigens, CD147 / genetics, metabolism Cartilage, Articular / enzymology Disease Models, Animal Disease Progression Enzyme Assays Gene Expression Regulation, Enzymologic / physiology Joint Instability / enzymology, etiology, physiopathology Knee Injuries / enzymology*, physiopathology Male Matrix Metalloproteinase 13 / genetics, metabolism Matrix Metalloproteinase 2 / genetics, metabolism Matrix Metalloproteinases / genetics, metabolism* Menisci, Tibial / enzymology Posterior Cruciate Ligament / enzymology RNA, Messenger / metabolism Rats Rats, Sprague-Dawley Synovial Membrane / enzymology Tissue Inhibitor of Metalloproteinase-1 / genetics, metabolism Tissue Inhibitor of Metalloproteinases / genetics, metabolism* Up-Regulation / physiology |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 0/Tissue Inhibitor of Metalloproteinase-1; 0/Tissue Inhibitor of Metalloproteinases; 136894-56-9/Antigens, CD147; EC 3.4.24.-/Matrix Metalloproteinase 13; EC 3.4.24.-/Matrix Metalloproteinases; EC 3.4.24.24/Matrix Metalloproteinase 2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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