Document Detail


Coordinated expression of MMPs and TIMPs in rat knee intra-articular tissues after ACL injury.
MedLine Citation:
PMID:  19863390     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Anterior cruciate ligament (ACL) has poor healing ability and an injured ACL would induce the degeneration of other intra-articular connective tissues. However, the coordinated expression and activities of matrix metalloproteinase (MMPs) in intra-articular tissues induced by ACL rupture were poorly understood. With a rat ACL rotating injury model, we found that after ACL injury, the mRNA levels of MMP-13, TIMP-1, and CD147 were significantly elevated in ACL, posterior cruciate ligament (PCL), synovium, meniscus, and cartilage. Also, MMP-2 activity was also elevated significantly in a time-dependent manner in all intra-articular tissues. Synovium showed the most capability to release MMPs, whereas ACL showed the highest MMP-13/TIMP-1 ratio. Generic MMP activity assay and zymography showed time dependent elevation of MMP activities in synovial fluids (SF). We concluded that the ACL injury would induce a coordinated response of intra-articular tissues to express MMPs, TIMPs, and CD147. The MMP activities in the microenvironment in SF would accumulate, released by all the intra-articular tissues, which would contribute to the knee damage and degeneration induced by ACL injury.
Authors:
Zhenyu Tang; Li Yang; Jin Zhang; Ruyue Xue; Yequan Wang; Peter C Chen; K-L Paul Sung
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Connective tissue research     Volume:  50     ISSN:  1607-8438     ISO Abbreviation:  Connect. Tissue Res.     Publication Date:  2009  
Date Detail:
Created Date:  2009-10-29     Completed Date:  2010-01-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0365263     Medline TA:  Connect Tissue Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  315-22     Citation Subset:  IM    
Affiliation:
Chongqing University, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anterior Cruciate Ligament / enzymology*,  injuries*,  physiopathology
Antigens, CD147 / genetics,  metabolism
Cartilage, Articular / enzymology
Disease Models, Animal
Disease Progression
Enzyme Assays
Gene Expression Regulation, Enzymologic / physiology
Joint Instability / enzymology,  etiology,  physiopathology
Knee Injuries / enzymology*,  physiopathology
Male
Matrix Metalloproteinase 13 / genetics,  metabolism
Matrix Metalloproteinase 2 / genetics,  metabolism
Matrix Metalloproteinases / genetics,  metabolism*
Menisci, Tibial / enzymology
Posterior Cruciate Ligament / enzymology
RNA, Messenger / metabolism
Rats
Rats, Sprague-Dawley
Synovial Membrane / enzymology
Tissue Inhibitor of Metalloproteinase-1 / genetics,  metabolism
Tissue Inhibitor of Metalloproteinases / genetics,  metabolism*
Up-Regulation / physiology
Chemical
Reg. No./Substance:
0/RNA, Messenger; 0/Tissue Inhibitor of Metalloproteinase-1; 0/Tissue Inhibitor of Metalloproteinases; 136894-56-9/Antigens, CD147; EC 3.4.24.-/Matrix Metalloproteinase 13; EC 3.4.24.-/Matrix Metalloproteinases; EC 3.4.24.24/Matrix Metalloproteinase 2

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