Document Detail


Coordinated reversal of flagellar motors on a single Escherichia coli cell.
MedLine Citation:
PMID:  21539787     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An Escherichia coli cell transduces extracellular stimuli sensed by chemoreceptors to the state of an intracellular signal molecule, which regulates the switching of the rotational direction of the flagellar motors from counterclockwise (CCW) to clockwise (CW) and from CW back to CCW. Here, we performed high-speed imaging of flagellar motor rotation and show that the switching of two different motors on a cell is controlled coordinatedly by an intracellular signal protein, phosphorylated CheY (CheY-P). The switching is highly coordinated with a subsecond delay between motors in clear correlation with the distance of each motor from the chemoreceptor patch localized at a cell pole, which would be explained by the diffusive motion of CheY-P molecules in the cell. The coordinated switching becomes disordered by the expression of a constitutively active CheY mutant that mimics the CW-rotation stimulating function. The coordinated switching requires CheZ, which is the phosphatase for CheY-P. Our results suggest that a transient increase and decrease in the concentration of CheY-P caused by a spontaneous burst of its production by the chemoreceptor patch followed by its dephosphorylation by CheZ, which is probably a wavelike propagation in a subsecond timescale, triggers and regulates the coordinated switching of flagellar motors.
Authors:
Shun Terasawa; Hajime Fukuoka; Yuichi Inoue; Takashi Sagawa; Hiroto Takahashi; Akihiko Ishijima
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biophysical journal     Volume:  100     ISSN:  1542-0086     ISO Abbreviation:  Biophys. J.     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-05-04     Completed Date:  2011-08-17     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  0370626     Medline TA:  Biophys J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2193-200     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Biophysical Society. Published by Elsevier Inc. All rights reserved.
Affiliation:
Graduate School of Life Sciences, Tohoku University, Aoba-ku, Sendai, Japan.
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MeSH Terms
Descriptor/Qualifier:
Artificial Cells / cytology,  metabolism
Bacterial Proteins / metabolism
Chemoreceptor Cells / cytology,  metabolism
Escherichia coli / cytology*,  metabolism*
Escherichia coli Proteins / metabolism
Flagella / metabolism*
Gene Deletion
Green Fluorescent Proteins / metabolism
Intracellular Space / metabolism
Membrane Proteins / metabolism
Models, Biological
Molecular Motor Proteins / metabolism*
Mutant Proteins / metabolism
Rotation
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Escherichia coli Proteins; 0/Membrane Proteins; 0/Molecular Motor Proteins; 0/Mutant Proteins; 0/methyl-accepting chemotaxis proteins; 147336-22-9/Green Fluorescent Proteins
Comments/Corrections

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