Document Detail


Coordinated regulation of lymph node vascular-stromal growth first by CD11c+ cells and then by T and B cells.
MedLine Citation:
PMID:  22031764     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lymph node blood vessels play important roles in the support and trafficking of immune cells. The blood vasculature is a component of the vascular-stromal compartment that also includes the lymphatic vasculature and fibroblastic reticular cells (FRCs). During immune responses as lymph nodes swell, the blood vasculature undergoes a rapid proliferative growth that is initially dependent on CD11c(+) cells and vascular endothelial growth factor (VEGF) but is independent of lymphocytes. The lymphatic vasculature grows with similar kinetics and VEGF dependence, suggesting coregulation of blood and lymphatic vascular growth, but lymphatic growth has been shown to be B cell dependent. In this article, we show that blood vascular, lymphatic, and FRC growth are coordinately regulated and identify two distinct phases of vascular-stromal growth--an initiation phase, characterized by upregulated vascular-stromal proliferation, and a subsequent expansion phase. The initiation phase is CD11c(+) cell dependent and T/B cell independent, whereas the expansion phase is dependent on B and T cells together. Using CCR7(-/-) mice and selective depletion of migratory skin dendritic cells, we show that endogenous skin-derived dendritic cells are not important during the initiation phase and uncover a modest regulatory role for CCR7. Finally, we show that FRC VEGF expression is upregulated during initiation and that dendritic cells can stimulate increased fibroblastic VEGF, suggesting the scenario that lymph node-resident CD11c(+) cells orchestrate the initiation of blood and lymphatic vascular growth in part by stimulating FRCs to upregulate VEGF. These results illustrate how the lymph node microenvironment is shaped by the cells it supports.
Authors:
Susan Chyou; Fairouz Benahmed; Jingfeng Chen; Varsha Kumar; Sha Tian; Martin Lipp; Theresa T Lu
Related Documents :
21814724 - Efficient production and characterization of recombinant human nell1 protein in human e...
21642954 - Regulated inactivation of the spindle assembly checkpoint without functional mitotic sp...
7718494 - Spatio-temporal receptive-field structure of phasic w cells in the cat retina.
22189964 - Electric impedance of human embryonic stem cell-derived retinal pigment epithelium.
11162524 - Two isoforms of trimming glucosidase ii exist in mammalian tissues and cell lines but n...
23959334 - Pgp 9.5 immunocytochemical staining for pancreatic endocrine tumors.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-10-26
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  187     ISSN:  1550-6606     ISO Abbreviation:  J. Immunol.     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-11-21     Completed Date:  2012-01-13     Revised Date:  2014-09-11    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5558-67     Citation Subset:  AIM; IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD11c / immunology
B-Lymphocytes / immunology*
Cell Separation
Cellular Microenvironment / immunology*
Chemotaxis, Leukocyte / immunology*
Dendritic Cells / immunology*
Endothelial Cells / immunology
Fibroblasts / immunology
Flow Cytometry
Lymph Nodes / blood supply*,  immunology
Lymphatic Vessels / immunology
Mice
Mice, Inbred C57BL
Mice, Knockout
NIH 3T3 Cells
T-Lymphocytes / immunology*
Vascular Endothelial Growth Factor A / immunology
Grant Support
ID/Acronym/Agency:
R01 AI068900/AI/NIAID NIH HHS; R01 AI068900-05/AI/NIAID NIH HHS; R01 AI079178/AI/NIAID NIH HHS; R01 AI079178/AI/NIAID NIH HHS; R01 AI079178-03/AI/NIAID NIH HHS; R01-AO068900/AO/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, CD11c; 0/Vascular Endothelial Growth Factor A
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Cutting edge: Calcium/Calmodulin-dependent protein kinase type IV is essential for mesangial cell pr...
Next Document:  Differential modulation of neurons in the rostral ventromedial medulla by neurokinin-1 receptors.