| Coordinate involvement of cysteine protease and nuclease in the executive phase of plant apoptosis. | |
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MedLine Citation:
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PMID: 15589846 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have developed an oat cell-free apoptosis system to investigate the execution mechanisms of plant apoptosis. Cell extracts derived from oat tissues undergoing toxin (victorin)-induced apoptosis caused nuclear collapse and internucleosomal DNA fragmentation in isolated nuclei. Pharmacological studies revealed that cysteine protease, which is E-64-sensitive but insensitive to caspase-specific inhibitors, is a crucial component in the morphological change of isolated nuclei, and that nuclease and the cysteine protease act cooperatively to induce the apoptotic DNA laddering. Interestingly, this finding is contrasted with those in well-studied animal cell-free systems in which an apoptotic endonuclease is solely responsible for the DNA fragmentation. |
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Authors:
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Koh Kusaka; Yasuomi Tada; Teppei Shigemi; Masaru Sakamoto; Hitoshi Nakayashiki; Yukio Tosa; Shigeyuki Mayama |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: FEBS letters Volume: 578 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 2004 Dec |
Date Detail:
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Created Date: 2004-12-13 Completed Date: 2005-01-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 363-7 Citation Subset: IM |
Affiliation:
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Graduate School of Science and Technology, Kobe University, Kobe 657-8501, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects,
physiology* Aurintricarboxylic Acid / pharmacology Avena sativa / cytology, enzymology*, metabolism Cell Extracts Cell Nucleus / drug effects Cell-Free System Cysteine Endopeptidases / drug effects, metabolism*, pharmacology Cysteine Proteinase Inhibitors / pharmacology DNA, Plant / drug effects Drug Interactions Endonucleases / metabolism*, pharmacology Leucine / analogs & derivatives*, pharmacology Protein Biosynthesis / drug effects Toxins, Biological / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Cell Extracts; 0/Cysteine Proteinase Inhibitors; 0/DNA, Plant; 0/Toxins, Biological; 4431-00-9/Aurintricarboxylic Acid; 61-90-5/Leucine; 66701-25-5/E 64; EC 3.1.-/Endonucleases; EC 3.4.22.-/Cysteine Endopeptidases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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