Document Detail

Coordinate expression of cyclooxygenase-2 and renin in the rat kidney in renovascular hypertension.
MedLine Citation:
PMID:  9453303     Owner:  NLM     Status:  MEDLINE    
Prostaglandins contribute to the regulation of renin synthesis and secretion. We tested the hypothesis that the inducible isoform of prostaglandin G/H synthase, cyclooxygenase-2, contributes to the stimulation of renin synthesis in renovascular hypertension. The expression of cyclooxygenase-2 and renin was investigated in the kidneys of rats with two-kidney, one-clip renovascular hypertension or sham operation. Systolic blood pressure was increased 2 weeks after clipping (153+/-7 versus 112+/-4 mmHg in controls, n=6 each, P<.05) and continued to rise until 4 weeks. Cyclooxygenase-2 mRNA levels were increased in clipped kidneys but remained unchanged or slightly decreased in nonclipped kidneys. Cyclooxygenase-2 protein was expressed mainly in the macula densa and occasionally in distal tubular cells not associated with the macula densa. Two weeks after clipping, the percentage of juxtaglomerular apparatus staining positive for cyclooxygenase-2 was 27.8+/-3.6 in clipped kidneys, 3.1+/-0.4 in contralateral kidneys, and 8.0+/-1.3 in controls; the percentages for immunoreactive renin staining in the afferent arteriole were 33.6+/-2 in clipped kidneys, 1.9+/-0.5 in contralateral kidneys, and 12.4+/-4.0 in controls, respectively. Similar parallel changes in renin and cyclooxygenase-2 staining were observed 4 weeks after clipping. The percentage of cyclooxygenase-2-positive juxtaglomerular apparatus correlated positively with the percentage that was renin positive (r=0.78, P<.05). Double immunostaining showed coexpression of cyclooxygenase-2 and renin protein in the same juxtaglomerular apparatus. Our data are consistent with a role for macula densa cyclooxygenase-2 in the regulation of renin in renovascular hypertension.
A Hartner; M Goppelt-Struebe; K F Hilgers
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Hypertension     Volume:  31     ISSN:  0194-911X     ISO Abbreviation:  Hypertension     Publication Date:  1998 Jan 
Date Detail:
Created Date:  1998-02-20     Completed Date:  1998-02-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7906255     Medline TA:  Hypertension     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  201-5     Citation Subset:  IM    
Department of Medicine IV, University of Erlangen-Nürnberg, Erlangen, Germany.
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MeSH Terms
Cyclooxygenase 2
Gene Expression Regulation, Enzymologic
Hypertension, Renovascular / enzymology*,  pathology
Isoenzymes / biosynthesis*
Kidney / enzymology*,  pathology
Prostaglandin-Endoperoxide Synthases / biosynthesis*
RNA, Messenger / biosynthesis
Rats, Sprague-Dawley
Reference Values
Renin / biosynthesis*
Transcription, Genetic
Reg. No./Substance:
0/Isoenzymes; 0/RNA, Messenger; EC 2; EC Synthases; EC

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