| Coordinate expression of cyclooxygenase-2 and renin in the rat kidney in renovascular hypertension. | |
MedLine Citation:
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PMID: 9453303 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Prostaglandins contribute to the regulation of renin synthesis and secretion. We tested the hypothesis that the inducible isoform of prostaglandin G/H synthase, cyclooxygenase-2, contributes to the stimulation of renin synthesis in renovascular hypertension. The expression of cyclooxygenase-2 and renin was investigated in the kidneys of rats with two-kidney, one-clip renovascular hypertension or sham operation. Systolic blood pressure was increased 2 weeks after clipping (153+/-7 versus 112+/-4 mmHg in controls, n=6 each, P<.05) and continued to rise until 4 weeks. Cyclooxygenase-2 mRNA levels were increased in clipped kidneys but remained unchanged or slightly decreased in nonclipped kidneys. Cyclooxygenase-2 protein was expressed mainly in the macula densa and occasionally in distal tubular cells not associated with the macula densa. Two weeks after clipping, the percentage of juxtaglomerular apparatus staining positive for cyclooxygenase-2 was 27.8+/-3.6 in clipped kidneys, 3.1+/-0.4 in contralateral kidneys, and 8.0+/-1.3 in controls; the percentages for immunoreactive renin staining in the afferent arteriole were 33.6+/-2 in clipped kidneys, 1.9+/-0.5 in contralateral kidneys, and 12.4+/-4.0 in controls, respectively. Similar parallel changes in renin and cyclooxygenase-2 staining were observed 4 weeks after clipping. The percentage of cyclooxygenase-2-positive juxtaglomerular apparatus correlated positively with the percentage that was renin positive (r=0.78, P<.05). Double immunostaining showed coexpression of cyclooxygenase-2 and renin protein in the same juxtaglomerular apparatus. Our data are consistent with a role for macula densa cyclooxygenase-2 in the regulation of renin in renovascular hypertension. |
Authors:
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A Hartner; M Goppelt-Struebe; K F Hilgers |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Hypertension Volume: 31 ISSN: 0194-911X ISO Abbreviation: Hypertension Publication Date: 1998 Jan |
Date Detail:
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Created Date: 1998-02-20 Completed Date: 1998-02-20 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7906255 Medline TA: Hypertension Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 201-5 Citation Subset: IM |
Affiliation:
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Department of Medicine IV, University of Erlangen-Nürnberg, Erlangen, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cyclooxygenase 2 Gene Expression Regulation, Enzymologic Hypertension, Renovascular / enzymology*, pathology Immunohistochemistry Isoenzymes / biosynthesis* Kidney / enzymology*, pathology Male Prostaglandin-Endoperoxide Synthases / biosynthesis* RNA, Messenger / biosynthesis Rats Rats, Sprague-Dawley Reference Values Renin / biosynthesis* Transcription, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Isoenzymes; 0/RNA, Messenger; EC 1.14.99.1/Cyclooxygenase 2; EC 1.14.99.1/Prostaglandin-Endoperoxide Synthases; EC 3.4.23.15/Renin |
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