| Cooperativity in the binding of the cationic biocide polyhexamethylene biguanide to nucleic acids. | |
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MedLine Citation:
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PMID: 15120614 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The interaction between the broad-spectrum antimicrobial agent, polyhexamethylene biguanide (PHMB), and various nucleic acids was investigated. Titration of either single- or double-stranded 100-bp DNA, or mixed-molecular weight marker DNA, or tRNA with PHMB caused precipitation of a complex between nucleic acid and PHMB in which the nucleotide/biguanide ratio was always close to unity. Binding of PHMB was highly cooperative, with apparent Hill coefficients 10.3-14.6. When a fluorescent derivative of PHMB was titrated with increasing amounts of nucleic acid, all four forms of nucleic acid caused strong polarisation of fluorescence, demonstrating the association with PHMB. The intensity and broad-spectrum binding of PHMB to all forms of nucleic acid has significant implications for the mechanism of action of this biocide. |
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Authors:
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Michael J Allen; Andrew P Morby; Graham F White |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 318 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2004 May |
Date Detail:
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Created Date: 2004-05-03 Completed Date: 2004-06-25 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 397-404 Citation Subset: IM |
Affiliation:
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Cardiff School of Biosciences, Cardiff University, Cardiff CF10 3US, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anti-Infective Agents
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chemistry,
metabolism Biguanides / chemistry, metabolism* Binding Sites Cations Chemical Precipitation DNA / chemistry, genetics, metabolism* Escherichia coli / genetics Fluorescence Polarization Kinetics RNA, Transfer / genetics, metabolism* Titrimetry / methods |
| Chemical | |
Reg. No./Substance:
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0/Anti-Infective Agents; 0/Biguanides; 0/Cations; 28757-47-3/polyhexamethylene biguanide; 9007-49-2/DNA; 9014-25-9/RNA, Transfer |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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