| Cooperation of hTERT, SV40 T antigen and oncogenic Ras in tumorigenesis: a cell transplantation model using bovine adrenocortical cells. | |
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MedLine Citation:
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PMID: 12407443 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Expression of TERT, the reverse transcriptase component of telomerase, is necessary to convert normal human cells to cancer cells. Despite this, "telomerization" by hTERT does not appear to alter the normal properties of cells. In a cell transplantation model in which bovine adrenocortical cells form vascularized tissue structures beneath the kidney capsule in scid mice, telomerization does not perturb the functional tissue-forming capacity of the cells. This cell transplantation model was used to study the cooperation of hTERT with SV40 T antigen (SV40 TAg) and oncogenic Ras in tumorigenesis. Only cells expressing all three genes were tumorigenic; this required large T, but not small t, antigen. These cells produced a continuously expanding tissue mass; they were invasive with respect to adjacent organs and eventually destroyed the kidney. Cells expressing only hTERT or only Ras produced minimally altered tissues. In contrast, SV40 TAg alone produced noninvasive nodules beneath the kidney capsule that had high proliferation rates balanced by high rates of apoptosis. The use of cell transplantation techniques in a cell type that is able to form tissue structures with or without full neoplastic conversion allows the phenotypes produced by individual cooperating oncogenes to be observed. |
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Authors:
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Michael Thomas; Tetsuya Suwa; Lianqing Yang; Lifang Zhao; Christina L Hawks; Peter J Hornsby |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Neoplasia (New York, N.Y.) Volume: 4 ISSN: 1522-8002 ISO Abbreviation: Neoplasia Publication Date: 2002 Nov-Dec |
Date Detail:
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Created Date: 2002-10-30 Completed Date: 2003-04-29 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 100886622 Medline TA: Neoplasia Country: United States |
Other Details:
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Languages: eng Pagination: 493-500 Citation Subset: IM |
Affiliation:
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Huffington Center on Aging, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX 77030, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adrenal Cortex
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cytology*,
metabolism,
transplantation Animals Antigens, Polyomavirus Transforming / physiology* Blotting, Western Cattle Cell Transformation, Neoplastic / metabolism, pathology* Cell Transformation, Viral / genetics Cell Transplantation Cells, Cultured / pathology DNA-Binding Proteins Female Humans Immunoenzyme Techniques Kidney Neoplasms / metabolism, pathology* Male Mice Mice, SCID Models, Biological Proto-Oncogene Proteins p21(ras) / physiology* Telomerase / physiology* Transfection |
| Grant Support | |
ID/Acronym/Agency:
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AG12287/AG/NIA NIH HHS; AG13663/AG/NIA NIH HHS; AG20752/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Polyomavirus Transforming; 0/DNA-Binding Proteins; EC 2.7.7.49/TERT protein, human; EC 2.7.7.49/Telomerase; EC 2.7.7.49/Tert protein, mouse; EC 3.6.5.2/HRAS protein, human; EC 3.6.5.2/Proto-Oncogene Proteins p21(ras) |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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