Document Detail

Cooperation of HDL Receptor and Hepatic Lipase in the Selective Uptake of HDL(2)-CE by Rat Hepatic Sinusoidal Cells.
MedLine Citation:
PMID:  12232594     Owner:  NLM     Status:  Publisher    
The recombined (3)H-CE-HDL(2)(rHDL(2)) keeps the biological activities of the native HDL(2). After rat hepatic sinusoidal cells were incubated with rHDL(2) at 37 degrees for 3 h (normal group), the cell-endocytic cpm was 995-/+147(mean-/+s, n=2). After the cells were further incubated for 2 h, the cell-release TCA-precipitable cpm and the TCA-supernatant cpm were 78-/+32 and 12-/+9 respectively. These values were 339-/+62, 19-/+11 and 9-/+5 respectively in the acetylimidazole-modified group, and 542-/+78, 34-/+14 and 9-/+8 respectively in the heparin-pretreated group. Our results suggested that: (1) Rat hepatic sinusoidal cells internalize HDL(2) and take up HDL(2)-CE by its HDL receptor, and HDL(3) was secreted out of the cells by retroendocytosis. (2) Hepatic lipase (HL) induces directly the selective uptake of HDL(2)-CE by the cells. (3) There is cooperation between the HDL receptor and HL in the selective uptake of HDL(2)-CE by the cells.
Qiang Wei; Man-Ping Wu; Pei-Fang Chen; Mei-Zhen Mei; Xin-Jian Gao
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Publication Detail:
Journal Detail:
Title:  Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica     Volume:  28     ISSN:  0582-9879     ISO Abbreviation:  Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao     Publication Date:  1996  
Date Detail:
Created Date:  2002-Sep-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  20730160R     Medline TA:  Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  659-664     Citation Subset:  -    
Department of Biochemistry, School of Pharmacy, Shanghai Medical University, Shanghai 200032, China.
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