| Cooling-induced contraction of the rat gastric fundus: mediation via transient receptor potential (TRP) cation channel TRPM8 receptor and Rho-kinase activation. | |
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MedLine Citation:
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PMID: 16173944 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. Cooling has been shown to induce contractions of several smooth muscles in vitro. However, the mechanism involved in the response is not yet known. In the present study, we investigated the possible involvement of transient receptor potential (TRP) cation channel TRPM8 receptors and the Rho-kinase pathway in cooling-induced contraction of the rat fundus. 2. Cooling-induced contractions were inversely proportional to temperature. Contractions were significantly reduced (by 65.6 +/- 2.4%; P < 0.05) in a Ca2+-free (1 mmol/L EGTA) medium, but were not significantly inhibited by nifedipine (10(-6) mol/L). 3. Capsazepine (3 x 10(-6) and 3 x 10(-5) mol/L), a TRPM8 receptor antagonist, inhibited cooling-induced contraction of the rat gastric fundus. 4. The Rho-kinase inhibitor Y-27632 concentration-dependently inhibited cooling-induced contraction of the gastric fundus, producing approximately 90% inhibition at a concentration of 10(-5) mol/L. Contractions were also inhibited by genistein (3 x 10(-5) mol/L), a tyrosine kinase inhibitor, but not by GF 109203X (10(-7) mol/L), a protein kinase C inhibitor. 5. Using reverse transcription-polymerase chain reaction techniques, it was observed that the mRNA for the TRPM8 receptor and Rho-kinase were expressed in the rat gastric fundus. 6. These results would suggest that cooling-induced contraction of the rat fundus is mediated by activation of TRPM8 receptors via a mechanism involving activation of Rho-kinase. |
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Authors:
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S Mustafa; Ma Oriowo |
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Publication Detail:
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Type: Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinical and experimental pharmacology & physiology Volume: 32 ISSN: 0305-1870 ISO Abbreviation: Clin. Exp. Pharmacol. Physiol. Publication Date: 2005 Oct |
Date Detail:
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Created Date: 2005-09-21 Completed Date: 2006-01-05 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0425076 Medline TA: Clin Exp Pharmacol Physiol Country: Australia |
Other Details:
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Languages: eng Pagination: 832-8 Citation Subset: IM |
Affiliation:
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Department of Nuclear Medicine, Faculty of Medicine, Kuwait University, Safat, Kuwait. seham@hsc.edu.kw |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amides
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pharmacology Animals Calcium / pharmacology Calcium Channel Blockers / pharmacology Capsaicin / analogs & derivatives, pharmacology Cold Temperature* Enzyme Activation Enzyme Inhibitors / pharmacology Gastric Fundus / drug effects, metabolism, physiology* Gene Expression / drug effects Genistein / pharmacology Indoles / pharmacology Intracellular Signaling Peptides and Proteins Male Maleimides / pharmacology Muscle Contraction / drug effects, physiology* Nifedipine / pharmacology Protein-Serine-Threonine Kinases / antagonists & inhibitors, metabolism Pyridines / pharmacology RNA, Messenger / genetics, metabolism Rats Rats, Sprague-Dawley TRPM Cation Channels / antagonists & inhibitors, genetics, physiology rho-Associated Kinases |
| Chemical | |
Reg. No./Substance:
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0/Amides; 0/Calcium Channel Blockers; 0/Enzyme Inhibitors; 0/Indoles; 0/Intracellular Signaling Peptides and Proteins; 0/Maleimides; 0/Pyridines; 0/RNA, Messenger; 0/TRPM Cation Channels; 0/Trpm8 protein, rat; 0/capsazepine; 133052-90-1/bisindolylmaleimide I; 138381-45-0/Y 27632; 21829-25-4/Nifedipine; 404-86-4/Capsaicin; 446-72-0/Genistein; 7440-70-2/Calcium; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/rho-Associated Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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