Document Detail


Conversion of xanthine dehydrogenase to oxidase in ischemic rat tissues.
MedLine Citation:
PMID:  3294898     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In response to global ischemia, tissue xanthine dehydrogenase was converted to xanthine oxidase in all tissues with half-times of conversion at 37 degrees C of approximately 3.6, 6, 7, and 14 h for the liver, kidney, heart, and lung, respectively. The time course of enzyme conversion at 4 degrees C was greatly extended with half-conversion times of 6, 5, 5, and 6 d for the respective tissues. Increases in xanthine oxidase activity were accompanied by the appearance of a distinct new protein species with greater electrophoretic mobility. The oxidase from ischemic rat liver was purified 781-fold and found to migrate with a higher mobility on native gels than the purified native dehydrogenase. Sodium dodecyl sulfate profiles revealed the presence of a single major band of 137 kD for the native dehydrogenase, whereas the oxidase had been partially cleaved generating polypeptides of 127, 91, and 57 kD. Polypeptide patterns for the oxidase resemble those seen following limited in vitro proteolysis of the native dehydrogenase supporting a proteolytic mechanism for the conversion of xanthine dehydrogenase to oxidase in ischemic rat liver.
Authors:
T D Engerson; T G McKelvey; D B Rhyne; E B Boggio; S J Snyder; H P Jones
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  The Journal of clinical investigation     Volume:  79     ISSN:  0021-9738     ISO Abbreviation:  J. Clin. Invest.     Publication Date:  1987 Jun 
Date Detail:
Created Date:  1987-07-10     Completed Date:  1987-07-10     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7802877     Medline TA:  J Clin Invest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1564-70     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Ischemia / enzymology*
Ketone Oxidoreductases / metabolism*
Kidney / enzymology
Liver / enzymology*
Lung / enzymology
Male
Myocardium / enzymology
Peptide Hydrolases / metabolism*
Protein Processing, Post-Translational
Rats
Rats, Inbred Strains
Temperature
Xanthine Dehydrogenase / metabolism*
Xanthine Oxidase / biosynthesis*,  isolation & purification
Chemical
Reg. No./Substance:
EC 1.17.1.4/Xanthine Dehydrogenase; EC 1.17.3.2/Xanthine Oxidase; EC 1.2.-/Ketone Oxidoreductases; EC 3.4.-/Peptide Hydrolases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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