Document Detail


Conversion of starch to ethanol in a recombinant Saccharomyces cerevisiae strain expressing rice alpha-amylase from a novel Pichia pastoris alcohol oxidase promoter.
MedLine Citation:
PMID:  7763608     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A recombinant Saccharomyces cerevisiae, expressing and secreting rice alpha-amylase, converts starch to ethanol. The rice alpha-amylase gene (OS103) was placed under the transcriptional control of the promoter from a newly described Pichia pastoris alcohol oxidase genomic clone. The nucleotide sequences of ZZA1 and other methanol-regulated promoters were analyzed. A highly conserved sequence (TTG-N3-GCTTCCAA-N5-TGGT) was found in the 5' flanking regions of alcohol oxidase, methanol oxidase, and dihydroxyacetone synthase genes in Pichia pastoris, Hansenula polymorpha, and Candida boidinii S2. The yeast strain containing the ZZA1-OS103 fusion secreted biologically active enzyme into the culture media while fermenting soluble starch.
Authors:
M H Kumagai; G G Sverlow; G della-Cioppa; L K Grill
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Bio/technology (Nature Publishing Company)     Volume:  11     ISSN:  0733-222X     ISO Abbreviation:  Biotechnology (N.Y.)     Publication Date:  1993 May 
Date Detail:
Created Date:  1993-06-04     Completed Date:  1993-06-04     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8309273     Medline TA:  Biotechnology (N Y)     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  606-10     Citation Subset:  B    
Affiliation:
Biosource Genetics Corporation, Vacaville, CA 95688.
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MeSH Terms
Descriptor/Qualifier:
Alcohol Oxidoreductases / genetics*
Amino Acid Sequence
Base Sequence
Cloning, Molecular
Conserved Sequence
DNA, Fungal / chemistry,  genetics
Escherichia coli / genetics
Ethanol / metabolism*
Gene Expression
Genes, Fungal
Molecular Sequence Data
Oryza sativa / enzymology*,  genetics
Pichia / enzymology,  genetics
Promoter Regions, Genetic
Saccharomyces cerevisiae / enzymology,  genetics,  metabolism*
Starch / metabolism*
Transformation, Bacterial
alpha-Amylases / genetics*,  metabolism
Chemical
Reg. No./Substance:
0/DNA, Fungal; 64-17-5/Ethanol; 9005-25-8/Starch; EC 1.1.-/Alcohol Oxidoreductases; EC 1.1.3.13/alcohol oxidase; EC 3.2.1.1/alpha-Amylases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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