Document Detail


Conversion of midbodies into germ cell intercellular bridges.
MedLine Citation:
PMID:  17383626     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Whereas somatic cell cytokinesis resolves with abscission of the midbody, resulting in independent daughter cells, germ cell cytokinesis concludes with the formation of a stable intercellular bridge interconnecting daughter cells in a syncytium. While many proteins essential for abscission have been discovered, until recently, no proteins essential for mammalian germ cell intercellular bridge formation have been identified. Using TEX14 as a marker for the germ cell intercellular bridge, we show that TEX14 co-localizes with the centralspindlin complex, mitotic kinesin-like protein 1 (MKLP1) and male germ cell Rac GTPase-activating protein (MgcRacGAP) and converts these midbody matrix proteins into stable intercellular bridge components. In contrast, septins (SEPT) 2, 7 and 9 are transitional proteins in the newly forming bridge. In cultured somatic cells, TEX14 can localize to the midbody in the absence of other germ cell-specific factors, suggesting that TEX14 serves to bridge the somatic cytokinesis machinery to other germ cell proteins to form a stable intercellular bridge essential for male reproduction.
Authors:
Michael P Greenbaum; Lang Ma; Martin M Matzuk
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-02-24
Journal Detail:
Title:  Developmental biology     Volume:  305     ISSN:  0012-1606     ISO Abbreviation:  Dev. Biol.     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-05-07     Completed Date:  2007-07-12     Revised Date:  2014-09-24    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  389-96     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
CHO Cells
Cricetinae
Cricetulus
Cytokinesis / physiology
Extracellular Space / enzymology,  physiology*
HeLa Cells
Humans
Intercellular Junctions / enzymology,  physiology*
Male
Mice
Organelles / enzymology,  physiology*
Protein-Serine-Threonine Kinases / physiology
Spermatozoa / cytology*,  enzymology,  metabolism
Subcellular Fractions / enzymology,  physiology
Transcription Factors / physiology
Grant Support
ID/Acronym/Agency:
F30MH066542/MH/NIMH NIH HHS; HD07495/HD/NICHD NIH HHS; T32GM07330/GM/NIGMS NIH HHS; U54 HD007495/HD/NICHD NIH HHS; U54 HD007495-350005/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/TEX14 protein, mouse; 0/Transcription Factors; EC 2.7.11.1/Protein-Serine-Threonine Kinases
Comments/Corrections

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