Document Detail

Conversion of the methionine hydroxy analogue DL-2-hydroxy-(4-methylthio) butanoic acid to sulfur-containing amino acids in the chicken small intestine.
MedLine Citation:
PMID:  17032826     Owner:  NLM     Status:  MEDLINE    
dl-Methionine or its corresponding hydroxy analogue, DL-2-hydroxy-(4-methylthio) butanoic acid (DLHMB), are commonly added to commercial animal diets to satisfy the TSAA requirement. The utilization of DLHMB as a supplementary source of Met begins with its conversion to L-Met via a 2-step mediated process. L-Methionine can then be transsulfurated to L-Cys, which, in turn, can be catabolized to taurine (TAU). In the present study, the capacity of the chicken small intestine to convert DLHMB to L-Met and to use this amino acid as a source for L-Cys and TAU production was evaluated. The appearance of Met in the serosal compartment of everted sacs incubated with DLHMB is higher in the presence of an H(+) gradient (mucosal pH 5.5 vs. 7.4). Serosal Cys and TAU concentration was compared in everted sacs incubated at a mucosal pH of 5.5 with DLHMB or L-Met, and the results show significantly higher values after incubation with the hydroxy analogue. Regional comparisons indicate no significant differences in the appearance of serosal Met and Cys, although lower values were obtained for TAU in the duodenum than in the jejunum and ileum. The profile of non-S amino acids was also determined and revealed no significant differences between DLHMB- and L-Met-incubated sacs. In conclusion, Cys and TAU content in chicken enterocytes is higher when DLHMB is used as a Met source.
R Martín-Venegas; P A Geraert; R Ferrer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Poultry science     Volume:  85     ISSN:  0032-5791     ISO Abbreviation:  Poult. Sci.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-10-11     Completed Date:  2006-12-19     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0401150     Medline TA:  Poult Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1932-8     Citation Subset:  IM    
Departament de Fisiologia, Facultat de Farmàcia, Universitat de Barcelona, 08028 Spain.
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MeSH Terms
Amino Acids, Sulfur / metabolism*
Animal Feed
Animal Nutritional Physiological Phenomena
Chickens / metabolism*
Cysteine / biosynthesis
Enterocytes / metabolism*
Hydrogen-Ion Concentration
Intestine, Small / metabolism*
Methionine / analogs & derivatives*,  metabolism
Nutritional Requirements
Random Allocation
Taurine / biosynthesis
Reg. No./Substance:
0/Amino Acids, Sulfur; 107-35-7/Taurine; 52-90-4/Cysteine; 583-91-5/alpha-hydroxy-gamma-methylmercaptobutyric acid; 63-68-3/Methionine

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