| Conversion of lipid transfer inhibitor protein (apolipoprotein F) to its active form depends on LDL composition. | |
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MedLine Citation:
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PMID: 21937674 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Lipid transfer inhibitor protein (LTIP) exists in both active and inactive forms. Incubation (37°C) of plasma causes LTIP to transfer from a 470kDa inactive complex to LDL where it is active. Here, we investigate the mechanisms underlying this movement. Inhibiting LCAT or cholesteryl ester transfer protein (CETP) reduced incubation-induced LTIP translocation by 40-50%. Blocking both LCAT and CETP completely prevented LTIP movement. Under appropriate conditions either factor alone could drive maximum LTIP transfer to LDL. These data suggest that chemical modification of LDL, the 470kDa complex, or both facilitate LTIP movement. To test this, LDL and the 470kDa fraction were separately premodified by CETP and/or LCAT activity. Modification of the 470kDa fraction had no effect on subsequent LTIP movement to native LDL. Premodification of LDL, however, induced spontaneous LTIP movement from the native 470kDa particle to LDL. This transfer depended on the extent of LDL modification and correlated negatively with changes in the LDL phospholipid + cholesterol to cholesteryl ester + triglyceride ratio. We conclude that LTIP translocation is dependent on LDL lipid composition, not on its release from the inactive complex. Compositional changes that reduce the surface to core lipid ratio of LDL promote LTIP binding and activation. |
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Authors:
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Richard E Morton; Diane J Greene |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-9-21 |
Journal Detail:
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Title: Journal of lipid research Volume: - ISSN: 0022-2275 ISO Abbreviation: - Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-9-22 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Cleveland Clinic Foundation, United States. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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