| Conversion of fumaric acid to L-malic by sol-gel immobilized Saccharomyces cerevisiae in a supported liquid membrane bioreactor. | |
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MedLine Citation:
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PMID: 12052057 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Conversion of fumaric acid (FA) to L-malic acid (LMA) was carried out in a bioreactor divided by two supported liquid membranes (SLMs) into three compartments: Feed, Reaction, and Product. The Feed/Reaction SLM, made of tri-n-octylphosphine oxide (vol 10%) in ethyl acetate, was selective toward the substrate, fumaric acid (S(FA/LMA) = 10). The Reaction/Product SLM, made of di(2-ethylhexyl) phosphate (vol 10%) in dichloromethane, was selective toward the product, L-malic acid (S(LMA/FA) = 680). Immobilized yeast engineered to overproduce the enzyme fumarase [E.C. 4.2.1.2] was placed in the Reaction compartment and served as the catalyst. The yeast was immobilized in small glasslike beads of alginate-silicate sol-gel matrix. The construction of the bioreactor ensured unidirectional flow of the substrate from the Feed to the Reaction and of the product from the Reaction to the Product compartments, with the inorganic counterion traveling in the opposite direction. The conversion of almost 100%, above the equilibrium value of ca. 84% and higher than that for the industrial process, 70%, was achieved. In contrast to the existing industrial biocatalytic process resulting in L-malic acid salts, direct production of the free acid is described. |
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Authors:
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Eyal Bressler; Ophry Pines; Israel Goldberg; Sergei Braun |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biotechnology progress Volume: 18 ISSN: 8756-7938 ISO Abbreviation: Biotechnol. Prog. Publication Date: 2002 May-Jun |
Date Detail:
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Created Date: 2002-06-07 Completed Date: 2002-12-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8506292 Medline TA: Biotechnol Prog Country: United States |
Other Details:
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Languages: eng Pagination: 445-50 Citation Subset: IM |
Affiliation:
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Department of Biological Chemistry, A. Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, 91904 Israel. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bioreactors* Catalysis Fumarate Hydratase / metabolism Fumarates / metabolism* Gels Malates / metabolism* Membranes, Artificial* Saccharomyces cerevisiae / enzymology, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Fumarates; 0/Gels; 0/Malates; 0/Membranes, Artificial; 110-17-8/fumaric acid; 6915-15-7/malic acid; EC 4.2.1.2/Fumarate Hydratase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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