| Conversion of big ET-1 in the rat lung: role of phosphoramidon-sensitive endothelin-1-converting enzyme. | |
| | |
MedLine Citation:
|
PMID: 8141343 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We examined conversion of Big endothelin-1 (ET-1) to mature ET-1 and pressor action during perfusion of the isolated perfused rat lung with Big ET-1. Big ET-1 caused a concentration-related increase in perfusion pressure and the pressor molar potency of the peptide was fivefold less than that of ET-1. Pressor responses to Big ET-1 were accompanied by an increase in immunoreactive-ET (IR-ET) levels in the perfusate and in the lung tissues. Pretreatment with phosphoramidon (10(-4) M), a metalloproteinase inhibitor, markedly suppressed the pressor action and increment in IR-ET in the tissues. Unexpectedly, the amount of IR-ET in the perfusate during perfusion of Big ET-1 was not influenced by phosphoramidon treatment. On the other hand, chymostatin, an inhibitor of chymotrypsin-like enzymes, effectively suppressed IR-ET levels in the perfusate; however, this enzyme inhibitor was without effect on the pressor action of Big ET-1 or on the increase in IR-ET levels in lung tissues. We tentatively conclude that the phosphoramidon-sensitive conversion of Big ET-T to ET-1 is linked to the pressor action of Big ET-1 in the isolated perfused rat lung. In addition, it seems likely that chymostatin-sensitive conversion of Big ET-1 to ET-1 does not play a major role in the conversion of the precursor to the mature form. We propose that IR-ET present in the tissues rather than that in the perfusate is a better indicator of the functional conversion of Big ET-1 in the rat lung. |
| | |
Authors:
|
K Hisaki; Y Matsumura; H Maekawa; K Fujita; M Takaoka; S Morimoto |
Related Documents
:
|
1810373 - Euro-collins solution versus uw-solution for long-term liver preservation in the isolat... 20732133 - Influence of oxygen supply on liver condition and elimination of dimethylacetamide in t... 9290463 - Plasma endothelin-1 and tumor necrosis factor-alpha concentrations in pregnant and cycl... |
Publication Detail:
|
Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The American journal of physiology Volume: 266 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1994 Feb |
Date Detail:
|
Created Date: 1994-04-26 Completed Date: 1994-04-26 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: H422-8 Citation Subset: IM |
Affiliation:
|
Department of Pharmacology, Osaka University of Pharmaceutical Sciences, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Aspartic Acid Endopeptidases / metabolism* Chymotrypsin / antagonists & inhibitors Dose-Response Relationship, Drug Endothelin-1 Endothelins / metabolism*, pharmacology* Glycopeptides / pharmacology* Kinetics Lung / drug effects, enzymology, physiology* Male Metalloendopeptidases / antagonists & inhibitors* Oligopeptides / pharmacology Perfusion Protein Precursors / metabolism Pulmonary Circulation / drug effects, physiology* Rats Rats, Sprague-Dawley |
| Chemical | |
Reg. No./Substance:
|
0/Endothelin-1; 0/Endothelins; 0/Glycopeptides; 0/Oligopeptides; 0/Protein Precursors; 36357-77-4/phosphoramidon; 9076-44-2/chymostatin; EC 3.4.21.1/Chymotrypsin; EC 3.4.23.-/Aspartic Acid Endopeptidases; EC 3.4.24.-/Metalloendopeptidases; EC 3.4.24.71/endothelin-converting enzyme |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Circulating neutrophil kinetics during tolerance in hemorrhagic shock using bacterial lipopolysaccha...
Next Document: Plasma norepinephrine variations correlate with peripheral vascular resistance in resting humans.