| A convenient dry feed for raising zebrafish larvae. | |
| | |
MedLine Citation:
|
PMID: 20441525 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Five protocols for raising zebrafish larvae were tested and compared in this study. These include four variations of feeding Fry Feed Kyowa N-250 (Kyowa) obtained from Miyako Kagaku, as well as an established protocol using Paramecia and supplemented with Zeigler dry feeds. The performance of these feeding protocols was evaluated using larvae obtained from two wild-type strains AB and AB/TL and two mutant strains gassi and smarca4(a50). Several quality parameters including survival rate, rearing density, growth rate, sex ratio, fecundity, eggs spawned by the resulting fish, and the corresponding survival rate were compared by logistic regression analyses. The results showed that the quality of AB and AB/TL larvae raised using one of the Kyowa feeding protocols was equally good when compared with the standard Paramecium protocol for up to 20 larvae/L. The protocol also provided a comparable but slightly lower survival rate for smarca4(a50) and inbred gassi lines, respectively. Nonetheless, all other quality parameters in the case of gassi were comparable to the established Paramecium protocol, even though the line carried multiple pigmentation mutations. Therefore, the optimized Kyowa feeding protocol can substantially reduce routine workload in maintaining a zebrafish research facility by virtually eliminating the need to culture live Paramecia feed. |
| | |
Authors:
|
Monica R Hensley; Yuk Fai Leung |
Related Documents
:
|
12081465 - Involvement of candida albicans nadh dehydrogenase complex i in filamentation. 8037745 - Induction of the putative copper atpases, copa and copb, of enterococcus hirae by ag+ a... 560475 - N-methyl-n'-nitro-n-nitrosoguanidine and uv induced mutants of the dinoflagellate crypt... 23090845 - Efficiency of different agrobacterium rhizogenes strains on hairy roots induction in so... 1514795 - Reassessment of the prevalence of heat-stable enterotoxin (nag-st) among environmental ... 7681525 - Genetic variations in baseline and ultraviolet light-induced sister chromatid exchanges... |
Publication Detail:
|
Type: Comparative Study; Journal Article |
Journal Detail:
|
Title: Zebrafish Volume: 7 ISSN: 1557-8542 ISO Abbreviation: Zebrafish Publication Date: 2010 Jun |
Date Detail:
|
Created Date: 2010-06-10 Completed Date: 2010-10-12 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101225070 Medline TA: Zebrafish Country: United States |
Other Details:
|
Languages: eng Pagination: 219-31 Citation Subset: IM |
Affiliation:
|
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animal Feed* Animal Husbandry / methods* Animal Nutritional Physiological Phenomena* Animals Fertility / physiology Larva / physiology Logistic Models Sex Ratio Zebrafish / growth & development* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Photoactivation of the CreER(T2) Recombinase for Conditional Site-Specific Recombination with High S...
Next Document: Management of facial nerve schwannoma: A single institution experience.