Document Detail

Controlling Pseudomonas aeruginosa persister cells by weak electrochemical currents and synergistic effects with tobramycin.
MedLine Citation:
PMID:  22840233     Owner:  NLM     Status:  Publisher    
It is well recognized that bacterial populations commonly contain a small percentage of phenotypic variants, known as persister cells, which are dormant and extremely tolerant to antibiotics. When the antibiotic treatment is stopped, surviving persister cells can regenerate the bacterial population with a similar percentage of persister cells. Such persistence presents a great challenge to curing chronic infections, such as those associated with implanted medical devices. In this study, we report that bacterial persister cells can be effectively eliminated by low-level direct currents (DCs); e.g. treatment with 70 μA/cm(2) DC for 1 h using stainless steel (SS) 304 reduced the number of viable planktonic persister cells of Pseudomonas aeruginosa PAO1 by 98% compared to the untreated control. In addition to persister killing by applying DC alone, synergistic effects were observed when treating persister cells with 70 μA/cm(2) DC and 1.5 μg/mL tobramycin together using SS304 electrodes. The same level of DC was also found to be cidal to biofilms-associated persister cells of P. aeruginosa PAO1. These results are helpful for developing more effective methods to control chronic infections associated with implanted medical devices.
Tagbo H R Niepa; Jeremy L Gilbert; Dacheng Ren
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-25
Journal Detail:
Title:  Biomaterials     Volume:  -     ISSN:  1878-5905     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Department of Biomedical and Chemical Engineering, Syracuse University, Syracuse, NY 13244, USA; Syracuse Biomaterials Institute, Syracuse University, Syracuse, NY 13244, USA.
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