Document Detail


Controlling proteome degradation in Daphnia pulex.
MedLine Citation:
PMID:  23027649     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An attempted proteomic study of Daphnia pulex response to sodium chloride exposure revealed an unexpected result: very strong proteome degradation previously observed in D. pulex is halted when animals have been exposed to elevated salinity prior to protein extraction. Further characterization of conditions controlling proteome degradation shows that the proteolytic factor(s) is/are present in an active form under chemically denaturing conditions in extract from control "normal/healthy" animals, and can be negatively regulated by prior in vivo salinity exposure of animals or inhibited by post-homogenization heating of samples. We interpret these data as evidence for salinity-induced inhibition of proteolysis in Daphnia.
Authors:
Catherine Jarrett Kemp; Dietmar Kültz
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-10-01
Journal Detail:
Title:  Journal of experimental zoology. Part A, Ecological genetics and physiology     Volume:  317     ISSN:  1932-5231     ISO Abbreviation:  J Exp Zool A Ecol Genet Physiol     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-19     Completed Date:  2013-04-18     Revised Date:  2013-12-04    
Medline Journal Info:
Nlm Unique ID:  101297745     Medline TA:  J Exp Zool A Ecol Genet Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  645-51     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
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MeSH Terms
Descriptor/Qualifier:
Animals
Daphnia / genetics*,  metabolism*
Electrophoresis, Gel, Two-Dimensional
Gene Expression Regulation / physiology
Proteome / chemistry*
Proteomics / methods*
Sodium Chloride
Grant Support
ID/Acronym/Agency:
P42 ES004699/ES/NIEHS NIH HHS; P42-ES004699/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Proteome; 7647-14-5/Sodium Chloride
Comments/Corrections

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