| Controlled release of IgG by novel UV induced polysaccharide/poly(amino acid) hydrogels. | |
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MedLine Citation:
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PMID: 19003848 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The development of new protein and peptide drugs needs new delivery systems able to entrap such drugs in safe conditions without affecting their structure and biological activity. In this context, the present work reports a new approach to load IgG, used as a model of therapeutic proteins such as anti-TNF-alpha monoclonal antibodies, into a polymeric system able to release the entrapped IgG in a controlled manner. In particular, new polysaccharide/poly(amino acid) UV induced hydrogels are proposed as colon delivery systems for human IgG. The poly(amino acid), alpha,beta-poly[N-(2-hydroxyethyl)-D,L-aspartamide], has been functionalized with methacrylic anhydride, while the polysaccharide, inulin, has been functionalized with methacrylic anhydride and succinic anhydride. The hydrogels were obtained by a short-time UV irradiation, in physiological-like conditions, without the use of radical initiators, at low temperature and in the presence or in the absence of PEGDM(550) used as a co-crosslinker in order to evaluate potential differences in terms of physicochemical properties and release profile. The obtained hydrogels were degradable by inulinase, showed a high cell compatibility and the released antibodies, analyzed by SEC and ELISA, retained their biological activity. |
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Authors:
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Giuseppe Tripodo; Giovanna Pitarresi; Gennara Cavallaro; Fabio Salvatore Palumbo; Gaetano Giammona |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Macromolecular bioscience Volume: 9 ISSN: 1616-5195 ISO Abbreviation: Macromol Biosci Publication Date: 2009 Apr |
Date Detail:
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Created Date: 2009-04-08 Completed Date: 2009-06-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101135941 Medline TA: Macromol Biosci Country: Germany |
Other Details:
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Languages: eng Pagination: 393-401 Citation Subset: IM |
Affiliation:
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Dipartimento di Chimica e Tecnologie Farmaceutiche, Università degli Studi di Palermo, Palermo, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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chemistry Caco-2 Cells Chromatography, Gel Crohn Disease / drug therapy* Delayed-Action Preparations / chemistry*, radiation effects Enzyme-Linked Immunosorbent Assay Humans Hydrogels / chemistry*, therapeutic use* Immunoglobulin G / chemistry* Polysaccharides / chemistry Ultraviolet Rays* |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Delayed-Action Preparations; 0/Hydrogels; 0/Immunoglobulin G; 0/Polysaccharides |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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