| Controlled release of 2, 3 desulfated heparin exerts its anti-inflammatory activity by effectively inhibiting E-selectin. | |
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MedLine Citation:
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PMID: 20540094 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Control of inflammation using appropriate anti-inflammatory agent prevents wound from becoming chronic. Heparin is a heterogeneous mixture of polysaccharide molecules with a mean molecular weight between 12-30 kDa containing 200-300 disaccharide units of glycosaminoglycan chains. Chemical modifications leading to generation of a unique pentasaccharide sequence effectively reduces its anticoagualant activity, while retaining its anti-inflammmatory property. In this study, Standard heparin was partially desulfated to 2, 3 desulfated heparin (2, 3 DSH) and its anti-inflammatory property was determined by assessing its ability to prevent static adhesion of leukocytes to endothelium and clotting assay. The effectiveness of 2, 3 DSH to down regulate E-selectin and key proinflammatory cytokines (IL-1beta and IL-6) was assessed by western blot and immunocytochemistry. These results were compared with commercially available 2-Desulfated Heparin (2DSH) and standard heparin (SH). Finally, a controlled delivery system of 2, 3 DSH was designed using chitosan microspheres and collagen as scaffold. Optimal loading of 2, 3 DSH was achieved and the release kinetics were tuned to follow a controlled release pattern. The steady state concentration of 2, 3 DSH was found to be optimal to elicit anti-inflammatory property and could achieve inhibition of E-selectin expression while unaffecting the normal clotting cascade. |
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Authors:
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T S Ramyaa Lakshmi; N Shanmugasundaram; S Shanmuganathan; K Karthikeyan; J Meenakshi; Mary Babu |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of biomedical materials research. Part A Volume: 95 ISSN: 1552-4965 ISO Abbreviation: J Biomed Mater Res A Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-08-26 Completed Date: 2010-12-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101234237 Medline TA: J Biomed Mater Res A Country: United States |
Other Details:
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Languages: eng Pagination: 118-28 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010. |
Affiliation:
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Biomaterials Division, Central Leather Research Institute, Chennai, Tamil Nadu, India. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anti-Inflammatory Agents
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chemistry,
pharmacology* Biological Assay Blood Coagulation / drug effects Blotting, Western Carbohydrate Conformation Chitosan / pharmacology Collagen / pharmacology Cytokines / metabolism Delayed-Action Preparations E-Selectin / metabolism* Endothelial Cells / cytology, metabolism Fluoresceins / metabolism Heparin / analogs & derivatives*, chemistry, pharmacology* Humans Inflammation Mediators / metabolism Magnetic Resonance Spectroscopy Microscopy, Electron, Scanning Microspheres Molecular Weight Partial Thromboplastin Time Reproducibility of Results Tissue Scaffolds |
| Chemical | |
Reg. No./Substance:
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0/Anti-Inflammatory Agents; 0/Cytokines; 0/Delayed-Action Preparations; 0/E-Selectin; 0/Fluoresceins; 0/Inflammation Mediators; 1461-15-0/fluorexon; 9005-49-6/Heparin; 9007-34-5/Collagen; 9012-76-4/Chitosan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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