| Controlled regional hypoperfusion in Langendorff heart preparations. | |
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MedLine Citation:
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PMID: 18256457 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We describe a new approach that combines several techniques to allow abnormal electrical and calcium activity to be visualized within hypoperfused myocardial tissue. A flexible microcannula was inserted into the left anterior descending artery of Langendorff perfused rat hearts, an air-tight seal between the coronary artery and the cannula was created, and an HPLC pump was used to deliver a specified flowrate through the microcannula. High resolution optical mapping of NADH/calcium, NADH/voltage or calcium/voltage was then conducted using a dual camera system. The ECG was acquired using surface electrodes. This perfusion technique is superior to occluding a vessel by either a tie or a clamp because it allows precise control of the composition and amount of flow to a defined ischemic bed. Another advantage is that flow can be stopped and resumed remotely, without touching the heart. This allows ectopic beats, or other arrhythmogenic activity, such as alternans, to be recorded immediately after changes in flow are imposed. Altogether, the described method provides a powerful new tool to assess how coronary flow rate affects the degree of local ischemia by the ability to record abnormal patterns of electrical activity and associated intracellular calcium transients with high spatiotemporal resolution from epicardial areas as small as 100 x 100 microm. |
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Authors:
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Luther Swift; Brian Martell; Vishal Khatri; Ara Arutunyan; Narine Sarvazyan; Matthew Kay |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2008-02-05 |
Journal Detail:
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Title: Physiological measurement Volume: 29 ISSN: 0967-3334 ISO Abbreviation: Physiol Meas Publication Date: 2008 Feb |
Date Detail:
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Created Date: 2008-02-07 Completed Date: 2008-04-30 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 9306921 Medline TA: Physiol Meas Country: England |
Other Details:
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Languages: eng Pagination: 269-79 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, The George Washington University, Washington, DC 20052, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Body Surface Potential Mapping / instrumentation*, methods Coronary Vessels / physiopathology* Electrocardiography / instrumentation*, methods Heart / physiopathology* Myocardial Reperfusion Injury / physiopathology* Organ Culture Techniques / instrumentation*, methods Perfusion / instrumentation*, methods Rats Systems Integration |
| Grant Support | |
ID/Acronym/Agency:
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HL076722/HL/NHLBI NIH HHS; R01 HL076722-01/HL/NHLBI NIH HHS; R01 HL076722-02/HL/NHLBI NIH HHS; R01 HL076722-03/HL/NHLBI NIH HHS; R01 HL076722-04/HL/NHLBI NIH HHS |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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