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Controlled production of proliferating somatic cell hybrids.
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MedLine Citation:
PMID:  4318843     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The techniques described permit the controlled production of large numbers of proliferating somatic cell hybrids in a relatively short period of time. Sendai virus is used to promote cell hybridization. beta-propriolactone is employed as the inactivating agent of Sendai virus since it produces complete loss of viral infectivity while preserving viral fusion capacity. Cells are fused in monolayer, instead of in suspension, since fixing cells in two dimensions permits one to control cell contacts during the fusion event through the expedient of varying multiplicities of the parental cells and the total cell density. Under the conditions described, a several hundred fold increase in the number of hybrid clones obtained is seen as compared to the controls.
Authors:
R J Klebe; T Chen; F H Ruddle
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of cell biology     Volume:  45     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1970 Apr 
Date Detail:
Created Date:  1970-11-06     Completed Date:  1970-11-06     Revised Date:  2010-06-22    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  74-82     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma
Animals
Cell Line / enzymology
Clone Cells
Culture Techniques*
Glucose-6-Phosphate Isomerase / metabolism
Hybridization, Genetic*
Karyotyping
Kidney
Mice
Mutation
Parainfluenza Virus 1, Human
Chemical
Reg. No./Substance:
EC 5.3.1.9/Glucose-6-Phosphate Isomerase
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Copyright © 1970 by The Rockefeller University Press
Received Day: 30 Month: 7 Year: 1969
Revision Received Day: 14 Month: 10 Year: 1969
Print publication date: Day: 1 Month: 4 Year: 1970
Volume: 45 Issue: 1
First Page: 74 Last Page: 82
ID: 2107999
PubMed Id: 4318843

CONTROLLED PRODUCTION OF PROLIFERATING SOMATIC CELL HYBRIDS
Robert J. Klebe
Tchaw-Ren Chen
Frank H. Ruddle
From the Department of Biology, Kline Biology Tower, Yale University, New Haven, Connecticut 06520


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