Document Detail

Controlled production of proliferating somatic cell hybrids.
Jump to Full Text
MedLine Citation:
PMID:  4318843     Owner:  NLM     Status:  MEDLINE    
The techniques described permit the controlled production of large numbers of proliferating somatic cell hybrids in a relatively short period of time. Sendai virus is used to promote cell hybridization. beta-propriolactone is employed as the inactivating agent of Sendai virus since it produces complete loss of viral infectivity while preserving viral fusion capacity. Cells are fused in monolayer, instead of in suspension, since fixing cells in two dimensions permits one to control cell contacts during the fusion event through the expedient of varying multiplicities of the parental cells and the total cell density. Under the conditions described, a several hundred fold increase in the number of hybrid clones obtained is seen as compared to the controls.
R J Klebe; T Chen; F H Ruddle
Related Documents :
1069993 - Coupling of catecholamine receptor from one cell with adenylate cyclase from another ce...
20083493 - Anti-glycophorin single-chain fv fusion to low-affinity mutant erythropoietin improves ...
15572683 - Growth rate and cell size modulate the synthesis of, and requirement for, g1-phase cycl...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of cell biology     Volume:  45     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1970 Apr 
Date Detail:
Created Date:  1970-11-06     Completed Date:  1970-11-06     Revised Date:  2010-06-22    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  74-82     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Line / enzymology
Clone Cells
Culture Techniques*
Glucose-6-Phosphate Isomerase / metabolism
Hybridization, Genetic*
Parainfluenza Virus 1, Human
Reg. No./Substance:
EC Isomerase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
Download PDF
Copyright © 1970 by The Rockefeller University Press
Received Day: 30 Month: 7 Year: 1969
Revision Received Day: 14 Month: 10 Year: 1969
Print publication date: Day: 1 Month: 4 Year: 1970
Volume: 45 Issue: 1
First Page: 74 Last Page: 82
ID: 2107999
PubMed Id: 4318843

Robert J. Klebe
Tchaw-Ren Chen
Frank H. Ruddle
From the Department of Biology, Kline Biology Tower, Yale University, New Haven, Connecticut 06520

Article Categories:
  • Article

Previous Document:  Serological studies with human papova (wart) virus.
Next Document:  The surface charge of rat liver mitochondria and their membranes. Clarification of some controversie...