| Controlled outgrowth of dissociated neurons on patterned substrates. | |
| | |
MedLine Citation:
|
PMID: 3054009 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The cytoarchitecture of nervous tissue is lost during the dissociation procedures used to form primary cell cultures. As a first step toward reestablishing an ordered arrangement of these cells in vitro, we developed a set of procedures for patterning the outgrowth of cells cultured on 2-dimensional substrates. These procedures used a combination of surface chemistry and photolithographic techniques. The adhesive properties of either silicon or silicon dioxide (quartz) surfaces were controlled by covalently binding small organic molecules to the surface with silane coupling agents. The attachment and growth of either embryonic mouse spinal cells or perinatal rat cerebellar cells were found to be promoted by binding certain amine derivatives to the surface. In particular, cells grown on surfaces bound with diamines and triamines, but not with monoamines, formed cultures whose morphology was similar to that of cells cultured on conventional substrates, i.e., glass coated with poly(D-lysine). The attachment of cells to a substrate was inhibited by binding alkane chains (e.g., n-tetradecane) to the surface and plating the cells in media containing 5-10% (vol/vol) serum. Patterns of selected adhesivity were formed using photochemical resist materials and lithographic masking techniques compatible with the silane chemistry. Cultures of either spinal cord cells or cerebellar cells could be confined to square regions on the scale of 50 micron. Cerebellar cells could be confined to grow on lines with widths less than 10 micron. This width is comparable to the diameter of granule cell somata. The patterned growth of cerebellar cells was maintained up to 12 d in vitro. Over this time period the granule cells were observed to develop electrical excitability and immunoreactivity for neuron-specific enolase. Purkinje neurons also developed electrical excitability when grown on the chemically modified surfaces. Immunochemical reactivity of the patterned cultures for glial fibrillary acid protein (GFAP) showed that glia are patterned along with the associated granule cells. Interestingly, the GFAP-positive glia that proliferated on surfaces bound with amine derivatives attained primarily a tile-shaped, fibroblast-like morphology, while those proliferating on glass coated with poly(D-lysine) developed primarily a spindle-shaped, process-bearing morphology. Granule cells preferentially associated with the spindle-shaped glia. |
| | |
Authors:
|
D Kleinfeld; K H Kahler; P E Hockberger |
Related Documents
:
|
20709949 - Neisseria lactamica selectively induces mitogenic proliferation of the naive b cell poo... 21193549 - Molecular characterization of propolis-induced cell death in saccharomyces cerevisiae. 1572319 - Resistance of hamster bronchiolar epithelium to neutrophil elastase: investigation by c... 3978639 - Effect of tunicamycin on release of macromolecules and tumor antigens by human melanoma... 21549109 - The unfolded protein response in human corneal endothelial cells following hypothermic ... 431729 - Thrombin-stimulated cell division involves proteolysis of its cell surface receptor. 20454659 - A comprehensive panel of three-dimensional models for studies of prostate cancer growth... 18000679 - In situ analysis by microspectroscopy reveals triterpenoid compositional patterns withi... 16625069 - Immunohistochemical distinction between merkel cell carcinoma and small cell carcinoma ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
|
Title: The Journal of neuroscience : the official journal of the Society for Neuroscience Volume: 8 ISSN: 0270-6474 ISO Abbreviation: J. Neurosci. Publication Date: 1988 Nov |
Date Detail:
|
Created Date: 1988-12-22 Completed Date: 1988-12-22 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8102140 Medline TA: J Neurosci Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 4098-120 Citation Subset: IM |
Affiliation:
|
Department of Molecular Biophysics, AT&T Bell Laboratories, Murray Hill, New Jersey 07974. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amines
/
pharmacology Amino Acids / pharmacology Animals Cell Adhesion / drug effects Cell Separation Cell Survival Cells, Cultured Cerebellum / cytology Cytological Techniques* Electrophysiology Ethylenediamines / pharmacology Glial Fibrillary Acidic Protein / analysis Immunohistochemistry Neurons / physiology* Phosphopyruvate Hydratase / analysis Polyamines / pharmacology Propylamines / pharmacology Purkinje Cells / physiology Silanes / pharmacology Surface Properties |
| Chemical | |
Reg. No./Substance:
|
0/Amines; 0/Amino Acids; 0/Ethylenediamines; 0/Glial Fibrillary Acidic Protein; 0/Polyamines; 0/Propylamines; 0/Silanes; 111-40-0/diethylenetriamine; EC 4.2.1.11/Phosphopyruvate Hydratase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Embryonic divergence of oligodendrocyte and astrocyte lineages in developing rat cerebrum.
Next Document: Effect of nimodipine on the outcome of patients after aneurysmal subarachnoid hemorrhage and surgery...