| Controlled light exposure microscopy reveals dynamic telomere microterritories throughout the cell cycle. | |
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MedLine Citation:
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PMID: 19097172 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Telomeres are complex end structures that confer functional integrity and positional stability to human chromosomes. Despite their critical importance, there is no clear view on telomere organization in cycling human cells and their dynamic behavior throughout the cell cycle. We investigated spatiotemporal organization of telomeres in living human ECV-304 cells stably expressing telomere binding proteins TRF1 and TRF2 fused to mCitrine using four dimensional microscopy. We thereby made use of controlled light exposure microscopy (CLEM), a novel technology that strongly reduces photodamage by limiting excitation in parts of the image where full exposure is not needed. We found that telomeres share small territories where they dynamically associate. These territories are preferentially positioned at the interface of chromatin domains. TRF1 and TRF2 are abundantly present in these territories but not firmly bound. At the onset of mitosis, the bulk of TRF protein dissociates from telomere regions, territories disintegrate and individual telomeres become faintly visible. The combination of stable cell lines, CLEM and cytometry proved essential in providing novel insights in compartment-based nuclear organization and may serve as a model approach for investigating telomere-driven genome-instability and studying long-term nuclear dynamics. |
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Authors:
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Winnok H De Vos; R A Hoebe; G H Joss; W Haffmans; S Baatout; P Van Oostveldt; E M M Manders |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cytometry. Part A : the journal of the International Society for Analytical Cytology Volume: 75 ISSN: 1552-4930 ISO Abbreviation: Cytometry A Publication Date: 2009 May |
Date Detail:
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Created Date: 2009-04-27 Completed Date: 2009-06-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101235694 Medline TA: Cytometry A Country: United States |
Other Details:
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Languages: eng Pagination: 428-39 Citation Subset: IM |
Copyright Information:
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(c) 2008 International Society for Advancement of Cytometry. |
Affiliation:
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Department of Molecular Biotechnology, Faculty of Bio-engineering Sciences, Ghent University, Coupure links 653, Ghent 9000, Belgium. winnok.devos@Ugent.be |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Cycle
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physiology* Cell Line Cell Line, Tumor Cell Nucleus / physiology Hela Cells Humans Microscopy, Fluorescence Recombinant Fusion Proteins / physiology Telomere / physiology* Telomeric Repeat Binding Protein 1 / physiology* Telomeric Repeat Binding Protein 2 / physiology* Transfection |
| Chemical | |
Reg. No./Substance:
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0/Recombinant Fusion Proteins; 0/TERF2 protein, human; 0/Telomeric Repeat Binding Protein 1; 0/Telomeric Repeat Binding Protein 2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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