| Controlled induction of distributed microdeformation in wounded tissue via a microchamber array dressing. | |
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MedLine Citation:
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PMID: 20607869 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mechanical stimuli are known to play an important role in determining the structure and function of living cells and tissues. Recent studies have highlighted the role of mechanical signals in mammalian dermal wound healing. However, the biological link between mechanical stimulation of wounded tissue and the subsequent cellular response has not been fully determined. The capacity for researchers to study this link is partially limited by the lack of instrumentation capable of applying controlled mechanical stimuli to wounded tissue. The studies outlined here tested the hypothesis that it was possible to control the magnitude of induced wound tissue deformation using a microfabricated dressing composed of an array of open-faced, hexagonally shaped microchambers rendered in a patch of silicone rubber. By connecting the dressing to a single vacuum source, the underlying wounded tissue was drawn up into each of the microchambers, thereby inducing tissue deformation. For these studies, the dressings were applied to full-thickness murine dermal wounds with 200 mmHg vacuum for 12 h. These studies demonstrated that the dressing was capable of inducing wound tissue deformation with values ranging from 11 to 29%. Through statistical analysis, the magnitude of the induced deformation was shown to be a function of both microchamber height and width. These results demonstrated that the dressing was capable of controlling the amount of deformation imparted in the underlying tissue. By allowing the application of mechanical stimulation with varying intensities, such a dressing will enable the performance of sophisticated mechanobiology studies in dermal wound healing. |
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Authors:
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Bartholomew J Kane; George Younan; Douglas Helm; Pouya Dastouri; Harrison Prentice-Mott; Daniel Irimia; Rodney K Chan; Mehmet Toner; Dennis P Orgill |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Journal of biomedical materials research. Part A Volume: 95 ISSN: 1552-4965 ISO Abbreviation: J Biomed Mater Res A Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-09-22 Completed Date: 2011-01-13 Revised Date: 2011-11-01 |
Medline Journal Info:
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Nlm Unique ID: 101234237 Medline TA: J Biomed Mater Res A Country: United States |
Other Details:
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Languages: eng Pagination: 333-40 Citation Subset: IM |
Affiliation:
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Division of Pediatric Surgery, Department of Surgery, University of Virginia Health System, Charlottesville, Virginia 22908, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bandages* Male Mice Mice, Inbred C57BL Microfluidics / instrumentation, methods Skin / pathology* Stress, Mechanical* Wound Healing* |
| Grant Support | |
ID/Acronym/Agency:
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P41 EB002503/EB/NIBIB NIH HHS; P41 EB002503-07/EB/NIBIB NIH HHS; R21 AR056446-01A1/AR/NIAMS NIH HHS; UL1RR 025758-01/RR/NCRR NIH HHS |
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