Document Detail


Controlled expression of the dominant flocculation genes FLO1, FLO5, and FLO11 in Saccharomyces cerevisiae.
MedLine Citation:
PMID:  18708514     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In many industrial fermentation processes, the Saccharomyces cerevisiae yeast should ideally meet two partially conflicting demands. During fermentation, a high suspended yeast count is required to maintain a satisfactory rate of fermentation, while at completion, efficient settling is desired to enhance product clarification and recovery. In most fermentation industries, currently used starter cultures do not satisfy this ideal, probably because nonflocculent yeast strains were selected to avoid fermentation problems. In this paper, we assess molecular strategies to optimize the flocculation behavior of S. cerevisiae. For this purpose, the chromosomal copies of three dominant flocculation genes, FLO1, FLO5, and FLO11, of the haploid nonflocculent, noninvasive, and non-flor-forming S. cerevisiae FY23 strain were placed under the transcriptional control of the promoters of the ADH2 and HSP30 genes. All six promoter-gene combinations resulted in specific flocculation behaviors in terms of timing and intensity. The strategy resulted in stable expression patterns providing a platform for the direct comparison and assessment of the specific impact of the expression of individual dominant FLO genes with regard to cell wall characteristics, such as hydrophobicity, biofilm formation, and substrate adhesion properties. The data also clearly demonstrate that the flocculation behavior of yeast strains can be tightly controlled and fine-tuned to satisfy specific industrial requirements.
Authors:
Patrick Govender; Jody L Domingo; Michael C Bester; Isak S Pretorius; Florian F Bauer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-08-15
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  74     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-09-26     Completed Date:  2008-10-23     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6041-52     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, University of KwaZulu-Natal, Private Bag X54001, Durban 4000, South Africa.
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MeSH Terms
Descriptor/Qualifier:
Alcohol Dehydrogenase / genetics
Biofilms / growth & development
Cell Wall / chemistry
Flocculation
Gene Expression Regulation, Fungal*
Glucose / metabolism
HSP30 Heat-Shock Proteins / genetics
Hydrophobic and Hydrophilic Interactions
Industrial Microbiology / methods
Lectins
Mannose / metabolism
Mannose-Binding Lectins
Membrane Glycoproteins
Membrane Proteins / biosynthesis*
Promoter Regions, Genetic
Saccharomyces cerevisiae / genetics,  physiology*
Saccharomyces cerevisiae Proteins / biosynthesis*,  genetics
Chemical
Reg. No./Substance:
0/FLO1 protein, S cerevisiae; 0/FLO5 protein, S cerevisiae; 0/HSP30 Heat-Shock Proteins; 0/HSP30 protein, S cerevisiae; 0/Lectins; 0/MUC1 protein, S cerevisiae; 0/Mannose-Binding Lectins; 0/Membrane Glycoproteins; 0/Membrane Proteins; 0/Saccharomyces cerevisiae Proteins; 31103-86-3/Mannose; 50-99-7/Glucose; EC 1.1.1.1/ADH2 protein, S cerevisiae; EC 1.1.1.1/Alcohol Dehydrogenase
Comments/Corrections

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