| Controlled cyclic stretch bioreactor for tissue-engineered heart valves. | |
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MedLine Citation:
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PMID: 19473698 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A tissue-engineered heart valve (TEHV) represents the ultimate valve replacement, especially for juvenile patients given its growth potential. To date, most TEHV bioreactors have been developed based on pulsed flow of culture medium through the valve lumen to induce strain in the leaflets. Using a strategy for controlled cyclic stretching of tubular constructs reported previously, we developed a controlled cyclic stretch bioreactor for TEHVs that leads to improved tensile and compositional properties. The TEHV is mounted inside a latex tube, which is then cyclically pressurized with culture medium. The root and leaflets stretch commensurately with the latex, the stretching being dictated by the stiffer latex and thus controllable. Medium is also perfused through the lumen at a slow rate in a flow loop to provide nutrient delivery. Fibrin-based TEHVs prepared with human dermal fibroblasts were subjected to three weeks of cyclic stretching with incrementally increasing strain amplitude. The TEHV possessed the tensile stiffness and stiffness anisotropy of leaflets from sheep pulmonary valves and could withstand cyclic pulmonary pressures with similar distension as for a sheep pulmonary artery. |
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Authors:
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Zeeshan H Syedain; Robert T Tranquillo |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural Date: 2009-05-26 |
Journal Detail:
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Title: Biomaterials Volume: 30 ISSN: 1878-5905 ISO Abbreviation: Biomaterials Publication Date: 2009 Sep |
Date Detail:
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Created Date: 2009-07-14 Completed Date: 2009-10-06 Revised Date: 2013-06-02 |
Medline Journal Info:
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Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
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Languages: eng Pagination: 4078-84 Citation Subset: IM |
Affiliation:
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Department of Chemical Engineering & Materials Science, University of Minnesota, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anisotropy Biocompatible Materials / metabolism Bioreactors* Cells, Cultured Culture Media / metabolism Extracellular Matrix / chemistry Fibroblasts / cytology Heart Valve Prosthesis* Heart Valves* Humans Materials Testing Sheep Stress, Mechanical Tensile Strength Tissue Culture Techniques* Tissue Engineering / instrumentation*, methods |
| Grant Support | |
ID/Acronym/Agency:
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HL71538/HL/NHLBI NIH HHS; R01 HL071538/HL/NHLBI NIH HHS; R01 HL071538-01/HL/NHLBI NIH HHS; R01 HL071538-02/HL/NHLBI NIH HHS; R01 HL071538-03/HL/NHLBI NIH HHS; R01 HL071538-04/HL/NHLBI NIH HHS; R01 HL071538-05/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Biocompatible Materials; 0/Culture Media |
| Comments/Corrections | |
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