Document Detail

Controlled cocultures of HeLa cells and human umbilical vein endothelial cells on detachable substrates.
MedLine Citation:
PMID:  19156292     Owner:  NLM     Status:  MEDLINE    
We investigated the interactions between HeLa cells and human umbilical vein endothelial cells (HUVECs) by monitoring their movements in a controllable coculture system. Two complementary, detachable, cell-substrates, one of polystyrene (PS) and the other of poly(dimethylsiloxane) (PDMS), were fabricated by replica molding. Coculturing was started by mechanically assembling two complementary substrates. One substrate was covered with a confluent layer of HeLa cells and its complement covered with confluent HUVECs. Using this coculture system as a tumor/endothelium model, we found that the HeLa cells migrated towards the HUVECs, while, simultaneously, the HUVECs retreated and that both types of cells migrated approximately twice as rapidly (two hundred microns per twenty-four hours) as they did alone. Additionally, when direct contact between the two cell types was prevented, the HUVECs initially migrated towards the HeLa cells and then retreated. The characteristics of the cell movements, i.e. direction and speed, probably are consequences of cell-cell signaling, with such signals possibly important during tumor cell intra- and extravasation.
Hirokazu Kaji; Takeshi Yokoi; Takeaki Kawashima; Matsuhiko Nishizawa
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-11-06
Journal Detail:
Title:  Lab on a chip     Volume:  9     ISSN:  1473-0197     ISO Abbreviation:  Lab Chip     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-21     Completed Date:  2009-05-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  England    
Other Details:
Languages:  eng     Pagination:  427-32     Citation Subset:  IM    
Department of Bioengineering and Robotics, Tohoku University, 6-6-01 Aramaki, Aoba-ku, Sendai 980-8579, Japan.
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MeSH Terms
Cell Communication
Cell Movement
Cells, Cultured
Coculture Techniques / methods*
Dimethylpolysiloxanes / chemistry
Endothelial Cells / physiology*
Endothelium, Vascular / cytology*,  physiology
Hela Cells
Microscopy, Fluorescence
Polystyrenes / chemistry
Signal Transduction
Umbilical Veins / cytology
Vascular Endothelial Growth Factor A / metabolism
Reg. No./Substance:
0/Dimethylpolysiloxanes; 0/Polystyrenes; 0/VEGFA protein, human; 0/Vascular Endothelial Growth Factor A; 63148-62-9/baysilon

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