| Controlled cell-seeding methodologies: a first step toward clinically relevant bone tissue engineering strategies. | |
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MedLine Citation:
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PMID: 20528665 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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The repair of large and complex bone defects could be helped by a cell-based bone tissue engineering strategy. A reliable and consistent cell-seeding methodology is a mandatory step in bringing bone tissue engineering into the clinic. However, optimization of the cell-seeding step is only relevant when it can be reliably evaluated. The cell seeding efficiency (CSE) plays a fundamental role herein. Results showed that cell lysis and the definition used to determine the CSE played a key role in quantifying the CSE. The definition of CSE should therefore be consistent and unambiguous. The study of the influence of five drop-seeding-related parameters within the studied test conditions showed that (i) the cell density and (ii) the seeding vessel did not significantly affect the CSE, whereas (iii) the volume of seeding medium-to-free scaffold volume ratio (MFR), (iv) the seeding time, and (v) the scaffold morphology did. Prolonging the incubation time increased the CSE up to a plateau value at 4 h. Increasing the MFR or permeability by changing the morphology of the scaffolds significantly reduced the CSE. These results confirm that cell seeding optimization is needed and that an evidence-based selection of the seeding conditions is favored. |
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Authors:
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Saartje Impens; Yantian Chen; Steven Mullens; Frank Luyten; Jan Schrooten |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-11 |
Journal Detail:
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Title: Tissue engineering. Part C, Methods Volume: 16 ISSN: 1937-3392 ISO Abbreviation: Tissue Eng Part C Methods Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101466663 Medline TA: Tissue Eng Part C Methods Country: United States |
Other Details:
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Languages: eng Pagination: 1575-83 Citation Subset: IM |
Affiliation:
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Material Technology, Flemish Institute for Technological Research (VITO), Boeretang, Mol, Belgium. jan.schrooten@med.kuleuven.be |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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