Document Detail


Controlled Synthesis of O-Glycopolypeptide Polymers and their Molecular Recognition by Lectins.
MedLine Citation:
PMID:  22497456     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The facile synthesis of high molecular weight water soluble O-glycopolypeptide polymers by the ring opening polymerization of their corresponding N-carboxyanhydride (NCA) in very high yield (over all yield >70%) is reported. The per-acetylated-O-glycosylated lysine-NCA monomers, synthesized using stable glycosyl donors and a commercially available protected amino acid in very high yield, was polymerized using commercially available amine initiators. The synthesized water soluble glycopolypeptides were found to be α-helical in aqueous solution. However, we were able to control the secondary conformation of the glycopolypeptides (α-helix versus non-helical structures) by polymerizing racemic amino acid glyco NCA's. We have also investigated the binding of the glycopolypeptide poly(α-manno-O-lys) with the lectin Con-A using precipitation and hemagglutination assays as well as by isothermal titration calorimetry (ITC). The ITC results clearly show that the binding process is enthalpy driven for both α-helical and non-helical structures, with negative entropic contribution. Binding stoichiometry for the glycopolypeptide poly(α-manno-O-lys) having a non-helical structure was slightly higher as compared to the corresponding polypeptide which adopted an α-helical structure.
Authors:
Debasis Pati; Ashif Y Shaikh; Soumen Das; Pavan Kumar Nareddy; Musti Joginadha Swamy; Srinivas Hotha; Sayam Sen Gupta
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-12
Journal Detail:
Title:  Biomacromolecules     Volume:  -     ISSN:  1526-4602     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100892849     Medline TA:  Biomacromolecules     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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