Document Detail


Control of starch composition and structure through substrate supply in the monocellular alga Chlamydomonas reinhardtii.
MedLine Citation:
PMID:  8663144     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In Chlamydomonas, as in higher plants, synthesis of ADP glucose catalyzed by ADP-glucose pyrophosphorylase is rate-limiting for the building of starch in the chloroplast. We have isolated disruptions of the STA1 ADP-glucose pyrophosphorylase structural gene that rendered the enzyme less responsive to the allosteric activator 3-phosphoglycerate. The structure and composition of the residual starch synthesized by all mutants of the STA1 locus is dramatically altered. The residual polysaccharide is shown to be devoid of amylose despite the presence of granule-bound starch synthase, the amylose biosynthetic enzyme. In addition, the fine structure of the mutant amylopectin revealed the presence of an altered chain-length distribution. This distribution mimicks that which is observed during growth and photosynthesis and differs markedly from that observed during storage. We therefore propose that low nucleotide sugar concentrations are either directly or indirectly responsible for the major differences observed in the composition or structure of starch during storage and photosynthesis.
Authors:
N Van den Koornhuyse; N Libessart; B Delrue; C Zabawinski; A Decq; A Iglesias; A Carton; J Preiss; S Ball
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  271     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1996 Jul 
Date Detail:
Created Date:  1996-08-29     Completed Date:  1996-08-29     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  16281-7     Citation Subset:  IM    
Affiliation:
Laboratoire de Chimie Biologique, Unité Mixte de Recherche du CNRS 111, Université des Sciences et Techniques de Lille Flandres-Artois 59655 Villeneuve d'Ascq Cedex, France 59655.
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MeSH Terms
Descriptor/Qualifier:
Amylopectin / isolation & purification,  metabolism
Amylose / isolation & purification,  metabolism
Animals
Base Sequence
Chlamydomonas reinhardtii / genetics,  growth & development,  metabolism*
Cloning, Molecular
DNA Primers
DNA, Plant / isolation & purification
Diploidy
Genes, Plant
Genotype
Glucose-1-Phosphate Adenylyltransferase
Haploidy
Kinetics
Molecular Sequence Data
Mutagenesis
Nucleotidyltransferases / biosynthesis,  genetics*,  metabolism
Polymerase Chain Reaction
Starch / biosynthesis*,  chemistry
Starch Synthase / metabolism*
Ultraviolet Rays
Grant Support
ID/Acronym/Agency:
AI 022385/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/DNA Primers; 0/DNA, Plant; 9005-25-8/Starch; 9005-82-7/Amylose; 9037-22-3/Amylopectin; EC 2.4.1.21/Starch Synthase; EC 2.7.7.-/Nucleotidyltransferases; EC 2.7.7.27/Glucose-1-Phosphate Adenylyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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