Document Detail

Control of replication of plasmid R1: translation of the 7k reading frame in the RepA mRNA leader region counteracts the interaction between CopA RNA and CopT RNA.
MedLine Citation:
PMID:  3556167     Owner:  NLM     Status:  MEDLINE    
Replication of IncFII plasmids is regulated through the expression of a gene, repA. The RepA protein is rate-limiting for initiation of replication. The main negative control is exerted by a countertranscript, CopA RNA, that binds to the complementary region of the RepA mRNA, thereby inhibiting the formation of the RepA protein. The target region for CopA RNA, CopT, is located upstream of the RepA coding region. An open reading frame for a putative 7k protein overlaps the CopT sequence. Here we show by using lacZ fusions that the 7k gene is expressed. We constructed a translation start mutation in order to abolish formation of the 7k protein. This resulted in a 10-fold decrease in repA expression. The 7k protein produced in trans did not reverse this effect, so the 7k protein per se does not control expression of repA. However, translation of the 7k coding sequence must influence CopA/CopT RNA recognition, since ribosomes will transiently disrupt the target hairpin. We propose here a novel mechanism that affects the level of gene expression: the 7k region of the RepA mRNA is a leader sequence that is involved in expression of the downstream gene; translation of the 7k region competes with a negative control system involving RNA-RNA interaction.
E G Wagner; J von Heijne; K Nordström
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The EMBO journal     Volume:  6     ISSN:  0261-4189     ISO Abbreviation:  EMBO J.     Publication Date:  1987 Feb 
Date Detail:
Created Date:  1987-07-06     Completed Date:  1987-07-06     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8208664     Medline TA:  EMBO J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  515-22     Citation Subset:  IM    
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MeSH Terms
Amino Acid Sequence
Bacterial Proteins / genetics*
Base Sequence
DNA Helicases*
DNA Replication*
DNA-Binding Proteins*
Escherichia coli / genetics*
Genes, Bacterial*
Protein Biosynthesis*
RNA, Messenger / genetics*
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA-Binding Proteins; 0/Proteins; 0/RNA, Messenger; 0/Trans-Activators; 0/replication initiator protein; EC 3.6.1.-/DNA Helicases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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