Document Detail


Control of protein structure and function through surface recognition by tailored nanoparticle scaffolds.
MedLine Citation:
PMID:  14733547     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Thioalkyl and thioalkylated oligo(ethylene glycol) (OEG) ligands with chain-end functionality were used to fabricate water-soluble CdSe nanoparticle scaffolds. Surface recognition of chymotrypsin (ChT) was achieved using these functionalized nanoparticle scaffolds, with three levels of interaction demonstrated: no interaction (OEG terminated with hydroxyl group), inhibition with denaturation (carboxylate-terminated thioalkyl ligands), and inhibition with retention of structure (carboxylate-terminated OEG). The latter process was reversible upon an increase in ionic strength, with essentially complete restoration of enzymatic activity.
Authors:
Rui Hong; Nicholas O Fischer; Ayush Verma; Catherine M Goodman; Todd Emrick; Vincent M Rotello
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of the American Chemical Society     Volume:  126     ISSN:  0002-7863     ISO Abbreviation:  J. Am. Chem. Soc.     Publication Date:  2004 Jan 
Date Detail:
Created Date:  2004-01-21     Completed Date:  2004-04-28     Revised Date:  2009-01-08    
Medline Journal Info:
Nlm Unique ID:  7503056     Medline TA:  J Am Chem Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  739-43     Citation Subset:  IM    
Affiliation:
Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, USA.
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MeSH Terms
Descriptor/Qualifier:
Cadmium Compounds / chemistry*
Chymotrypsin / antagonists & inhibitors,  chemistry*,  metabolism
Circular Dichroism
Fatty Acids / chemistry
Nanotechnology / methods*
Osmolar Concentration
Polyethylene Glycols / chemistry*
Protein Conformation
Selenium Compounds / chemistry*
Surface Properties
Grant Support
ID/Acronym/Agency:
GM 62998/GM/NIGMS NIH HHS; GM08515/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Cadmium Compounds; 0/Fatty Acids; 0/Polyethylene Glycols; 0/Selenium Compounds; 112-37-8/undecanoic acid; 1306-24-7/cadmium selenide; EC 3.4.21.-/alpha-chymotrypsin; EC 3.4.21.1/Chymotrypsin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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